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化工学报  2014 

超大孔聚丙烯酰胺晶胶微球的制备及其生物相容性和吸附性能

DOI: 10.3969/j.issn.0438-1157.2014.06.054, PP. 2350-2356

Keywords: 多孔介质,聚合物,晶胶微球,离子交换,原位接枝,生物相容性,吸附

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Abstract:

利用反相悬浮结合溶剂结晶致孔法制备了具有超大孔隙、球形度良好的聚丙烯酰胺晶胶微球,晶胶微球平均粒径为234.1mm,孔径约为10~50mm。采用原位接枝法,将2-丙烯酰胺基-2-甲基-1-丙烷磺酸(AMPSA)接枝到晶胶微球孔隙表面上,得到了带有磺酸基团的阳离子交换晶胶微球。考察了晶胶微球的生物相容性和吸附蛋白质及重金属离子Cu2+的性能,结果表明:在大肠杆菌培养液中,添加晶胶微球,对大肠杆菌的生长影响不大。接枝了AMPSA的晶胶微球表现出更强的吸附Cu2+的能力,吸附容量达到1.14mmol·g-1。同时,接枝后的晶胶微球也具有一定吸附蛋白质的能力,溶菌酶的吸附容量达到54.5mg·g-1。因此,该大孔晶胶可望在微生物固定化、生物分离和重金属离子吸附中会发挥更大的作用。

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