全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
化工学报  2015 

高分子大孔微球的制备和结构控制

DOI: 10.11949/j.issn.0438-1157.20150948, PP. 2846-2853

Keywords: 大孔微球,结构控制,药物缓控释,分离纯化

Full-Text   Cite this paper   Add to My Lib

Abstract:

高分子大孔微球的形成和结构调控是一个复杂的过程,特别对于孔径超过100nm的超大孔微球而言,无法通过常规的制孔剂与聚合物之间的相分离得到。针对生化工程对于超大孔微球的重要需求,发展了反胶团溶胀法和复乳法,实现了孔径在百纳米级以上的控制。研究了微球结构对于应用效果的影响。超大孔微球用于生物大分子的分离纯化,显示出高载量、高活性回收率、高纯化倍数的特点。超大孔微球固定化酶在酶的热稳定性、储存稳定性、重复使用性能等方面具有显著的优势。微球的结构有效控制是在不同领域获得成功应用的保障。

References

[1]  Qu J B, Zhou W Q, Wei W, Su Z G, Ma G H. Chemical modification and characterization of gigaporous polystyrene microspheres as rapid separation of proteins base supports [J]. J. Polym. Sci. Pol. Chem., 2008, 46(17): 5794-5804.
[2]  Qu J B, Zhou W Q, Su Z G, Ma G H. Preparation and characterization of gigaporous DEAE-polystyrene anion exchange resin as high-speed protein chromatography medium [J]. J. Biotechnol., 2008, 136(S1): 452-453.
[3]  Yu M R, Li Y, Zhang S P, Li X N, Yang Y L, Chen Y, Ma G H, Su Z G. Improving stability of virus-like particles by ion-exchange chromatographic supports with large pore size: advantages of gigaporous media beyond enhanced binding capacity [J]. J. Chromatogr. A, 2014, 1343: 109-118.
[4]  Li Y, Gao F, Wei W, Qu J B, Ma G H, Zhou W Q. Pore size of macroporous polystyrene microspheres affects lipase immobilization [J]. J. Mol. Catal. B: Enzymatic, 2010, 66 (1/2): 182-189.
[5]  Zhou W Q, Gu T Y, Su Z G, Ma G H. Synthesis of macroporous poly(styrene-divinyl benzene) microspheres by surfactant reverse micelles swelling method [J]. Polymer, 2007, 48(7): 1981-1988.
[6]  Zhou W Q, Gu T Y, Su Z G, Ma G H. Synthesis of macroporous poly(glycidyl methacrylate) microspheres by surfactant reverse Micelles swelling method [J]. Eur. Polym. J., 2007, 43(10): 4493-4502.
[7]  Zhou W Q, Li J, Wei W, Su Z G, Ma G H. Effect of solubilization of surfactant aggregates on pore structure in gigaporous polymeric particles [J]. Colloids and Surfaces A: Physicochem. Eng. Aspects., 2011, 384 (1/3): 549-554.
[8]  Na X M, Gao F, Zhang L Y, Su Z G, Ma G H. Biodegradable microcapsules prepared by self-healing of porous microspheres [J]. ACS Macro Letters, 2012, 1(6): 697-700.
[9]  Zhai Y Q, Zhou W Q, Wei W, Qu J B, Lei J D, Su Z G, Ma G H. Functional gigaporous polystyrene microspheres facilitating separation of poly(ethylene glycol)-protein conjugate [J]. Analytica Chimica Acta, 2011, 712: 152-161.
[10]  Zhang R Y, Li Q, Gao Y, Li J, Huang Y D, Song C, Zhou W Q, Ma G H, Su Z G. Hydrophilic modification gigaporous resins with poly(ethylenimine) for high-throughput proteins ion-exchange chromatography [J]. J. Chromatogr. A, 2014, 1343: 109-118.
[11]  Zhang R Y, Li Q, Gao Y, Li J, Zhou W Q, Ye P L, Gao Y, Ma G H, Su Z G. Covalently coating dextran on macroporous polyglycidyl methacrylate microsphere enabled rapid protein chromatographic separation [J]. Materials Sci. Engineer: C, 2012, 32(8): 2628-2633.
[12]  Qu J B, Zhou W Q, Wei W, Su Z G, Ma G H. An effective way to hydrophilize gigaporous polystyrene microspheres as rapid chromato-graphic separation media for proteins [J]. Langmuir, 2008, 24(23): 13646-13652.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133