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化工学报  2015 

ORP对酿酒酵母在木质纤维素水解液抑制物中发酵的影响

DOI: 10.11949/j.issn.0438-1157.20141318, PP. 1066-1071

Keywords: 木质纤维素,抑制物,胁迫耐受性,氧化还原电位,脱毒

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Abstract:

木质纤维素水解液中的毒性副产物对酿酒细胞具有抑制作用,采用氧化还原电位(oxidoreductionpotential,ORP)调控,能够提高细胞对抑制物的耐受性。考察了代表性抑制物乙酸、糠醛及苯酚对酵母细胞生长和代谢的影响,并通过添加氧化剂赤血盐(K3[Fe(CN)6])和还原剂二硫苏糖醇(DTT)调节发酵液ORP初始值在(305±5)mV、(157±8)mV及(-150±5)mV,研究不同ORP条件下细胞对抑制物的耐受性影响。结果表明,氧化态能提高生物量并缩短发酵时间。除苯酚外,氧化态也利于提高乙醇的收率。对细胞保护剂甘油合成的方面,氧化态和还原态都会促进甘油合成。在水解液脱毒方面,氧化态表现比还原态要好,因为氧化态促进生物量积累,有利于通过细胞自身代谢脱毒。

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