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化工学报  2015 

F/F对嗜盐污泥以乙酸钠为底物生产PHB能力的影响

DOI: 10.11949/j.issn.0438-1157.20141149, PP. 1491-1497

Keywords: 聚-b-羟基脂肪酸酯,嗜盐活性污泥,两步法,PHA合成菌,菌群选择,生物过程,生物技术,数值分析

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Abstract:

嗜盐混合菌生产PHB具有较好的成本优势。本研究接种入海口底泥发展嗜盐活性污泥,利用嗜盐活性污泥以两步法工艺生产PHB。在连续300d的试验中从长期试验、发酵生产PHB以及表观动力学等方面探索了不同盛宴期/饥饿期(F/F)下筛选菌群的PHB积累能力。研究发现F/F作为重要的参数直接影响嗜盐活性污泥的PHB积累能力。在高F/F下非PHB积累菌群成为优势菌,而低F/F下促进高聚PHB嗜盐菌的选择和富集。在F/F≤0.33条件下筛选的嗜盐活性污泥最大PHB含量可以达到细胞干重的46.7%,PHB产率为0.358mgPHB·(mgAc)-1。研究结果将对进一步实现嗜盐污泥生产PHB提供有益的指导。

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