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化工学报  2015 

紫外光谱定量测定木质纤维预水解液中溶解性木素和糠醛含量

DOI: 10.11949/j.issn.0438-1157.20150012, PP. 2295-2302

Keywords: 紫外光谱,生物质,水解,木素,降解,糠醛

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Abstract:

采用紫外光谱探讨了低分子有机酸、糠醛和木质纤维高温预水解液(PHL)的紫外吸收谱图特征。甲酸和乙酸在近205nm处有显著吸收,而糠醛在近280nm处有显著吸收,以205nm或280nm为木素特征吸收位置的传统木素测定方法不适于高温预水解液的溶解性木素测定。研究提出碱性硼氢化钠还原处理预水解液,消除糠醛的紫外吸收,进而定量测定溶解性木素含量的方法。结果表明,硼氢化钠可显著降低近280nm处吸收值,预水解条件越高,还原前后吸收差值降低越多,说明糠醛类物质含量高。以提取相思木和芦苇木素为标样,280nm处吸光度与浓度线性相关系数均达到0.999,满足定量测定的需要。充分还原前后280nm吸收差值可用于糠醛的定量,糠醛标样280nm处吸光度与浓度线性相关性为0.998,糠醛测定回收率为98.14%~99.88%,相对标准偏差为0.17%~0.35%。

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