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化工学报  2015 

大肠杆菌温敏耐热系统的构建与应用

DOI: 10.11949/j.issn.0438-1157.20141929, PP. 2613-2619

Keywords: 生物技术,合成生物学,发酵,耐热性,RNA温敏开关,热激蛋白,赖氨酸

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Abstract:

发酵过程中热胁迫不仅影响微生物的生长和生产,还因冷却控温增加了生产成本。通过人工设计合成的温敏型RNA开关调控来源于腾冲嗜热菌(ThermoanaerobactertengcongensisMB4)的热激蛋白DnaK的表达,该温敏型耐热系统显著提高了大肠杆菌的耐热性,同时还减少了大肠杆菌在37℃过表达热激蛋白的代谢负荷。将该系统应用于产赖氨酸大肠杆菌的高温发酵,不仅强化了其在40℃下的生长能力,而且显著提高了其在高温下的生产能力,赖氨酸产量比对照组提高了2.95倍。温敏型耐热系统的应用为人工耐热生物系统的构建提供了新方法。

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