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核农学报  2011 

空间诱变水稻多蘖矮突变体不同分蘖期蛋白质组研究

, PP. 405-415

Keywords: 水稻,多蘖矮,空间诱变,蛋白质组,S-类核糖核酸酶

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Abstract:

JB-1返回式卫星搭载水稻(OryzasativaL.)丙95-503干种子,地面种植后筛选出多蘖矮杆突变体R955。利用荧光差异显示双向电泳(2-DDIGE)对突变体播种后第14天(未发生分蘖)、第21天(分蘖起始)、第55天(最高分蘖期)3个营养生长期叶片总蛋白进行分离及定量分析(未搭载植株为对照),检测到在各发育时期共有97个蛋白点发生差异表达,经串联质谱鉴定后得到59个独特的蛋白。功能分析发现能量代谢、光合作用、蛋白代谢、氮元素同化、氨基酸代谢以及胁迫应答等过程均参与了突变体的分蘖发育。对差异蛋白在不同时期相对表达量的K-均值聚类分析显示不同功能蛋白表现不同表达模式。两因素差分析(Two-wayANOVA)筛选出S-类核糖核酸酶可能与水稻分蘖性状直接关联。

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