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核农学报  2011 

EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析

Keywords: 小麦,甲基磺酸乙酯(EMS),突变体,小麦黄矮病抗性

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Abstract:

抗黄矮病的小麦-中间偃麦草易位系YW642携带中间偃麦草抗黄矮病基因Bdv2。用化学诱变剂甲基磺酸乙酯(EMS)处理抗黄矮病的小麦-中间偃麦草易位系YW642的种子6000粒,从M2中筛选出32类不同性状的突变体73株,表型变异率约为7.38%。对感黄矮病突变体的M3、M4代继续进行鉴定,证明18个感黄矮病突变体的突变性状可以遗传,其黄矮病抗性丧失程度不等。用分子标记对上述感黄矮病突变体及其对照进行分析,结果发现这些突变体分别在1-4个分子标记位点上发生变异,说明这些突变体中Bdv2及其附近区域有不同碱基位点发生突变。该研究创造的YW642的突变体,为小麦抗黄矮病基因克隆和功能基因组学研究奠定了坚实的材料基础。

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