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核农学报  2012 

红外光谱法研究铬对酵母细胞内蛋白二级结构的影响

, PP. 505-510

Keywords: 红外光谱,,二级结构,葡萄糖耐量因子,酵母

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Abstract:

利用氨水、SephadexG-75对富铬酵母、空白酵母进行蛋白提取,采用红外光谱测定及数学方法对比分析铬作用前后蛋白质二级结构的变化,探讨铬对蛋白结构的影响。结果显示,铬与蛋白的键合导致大分子蛋白中的α-螺旋结构和无规则卷曲结构被破坏,α-螺旋结构由11.35%降至1.63%,无规则卷曲结构略有下降,β-转角结构增加,由33.88%升至52.72%,β-折叠结构几乎无变化;小分子蛋白的β-折叠结构被破坏,由61.41%降至48.54%,无规则卷曲和α-螺旋结构比例增加,由1.41%增加至13.30%,α-螺旋结构略有增加,β-转角结构几乎无变化。与铬结合的大分子蛋白中,β-转角结构占优势;与铬结合的小分子蛋白中,β-折叠结构占优势。

References

[1]  王建华,卫亚丽,文宗河,何建川.蛋白质结构的FT-IR研究进展[J].化学通报, 2004, (7):482-486
[2]  刘 鹭,吕加平,高艳红.空间搭载高产葡萄糖耐量因子(GTF)酵母的选育[J].微生物学通报, 2009, 36 (2):223-230
[3]  Anderso RA, Polansky M M, Roginski E E, et al. Factors affecting the retention and extraction of yeast chromium [J]. Journal of Agriculture and Food Chemistry, 1978, 26 (4):858-861
[4]  Mertz W. Effects and metabolism of glucose tolerance factor [J]. Nutrition Reviews. 1975,33 (5):129-135
[5]  王 翀.啤酒酵母对重金属Cr(Ⅲ)生物转化及生物吸附的研究[D].西安:西北大学, 2005
[6]  GB/T15555.6-1995,固体废物总铬的测定[S]
[7]  Sumral K H, Vincent J B. Is glucose tolerance factor an artifact produced by acid hydrolysis of low-molecular-weight chromium-binding substance [J]. Polyhedron, 1997, 16 (23): 4171-4177
[8]  Mertz W. The role of elements in the aging process [M]. Progress in Clinical and Biological Research, 1990,326:229-240
[9]  Holm R H, Kennepo P, Solomon E I. Structural and functional aspects of metal sites in biology [J]. Chemical Reviews,1996, 96: 2239-2314
[10]  Liu C, Xu H J. The metal site as a template for the metalloprotein structure formation [J]. Journal of Inorganic Biochemistry, 2002, 88: 77-86
[11]  Zetic V G, Stehlik-tomas V, Grba S, et al. Chromium uptake by Sacchaomyces cerevisiae and isolation of glucose tolerance factor from yeast biomass [J]. Journal of Bioscience, 2001, 26 (2):217-223
[12]  Toepfer E W, Mertz W, Polansky M M, et al. Preparation of chromium-containing material of glucose tolerance factor activity from brewer’s yeast extracts and by synthesis [J]. Journal of Agriculture and Food Chemistry, 1976, 25 (1):162-166.
[13]  刘 鹭,吕加平,高艳红. 酵母源性葡萄糖耐量因子(GTF)含量测定方法的研究[J].食品与发酵工业, 2009, 35 (1):140-144
[14]  Edens N K, Reave L A, Bergana M S, et al. Yeast extract stimulates glucose metabolism and inhibits lipolysis in rat adipocytes in vitro [J]. Nutritionr. 2002, 132:1141-1148
[15]  Davis D M, Holdsworth E S, Sherriff J L. The isolation of glucose tolerance factors from brewer’s yeast and their relationship to chromium [J]. Journal of Biochmeical Medicine, 1985,33:297-311
[16]  Haylock S J, Buckley P D, Blackwellel L F. Separation of biologically active chromium~containing complexes from yeast extracts and other sources of glucose tolerance factor(GTF) activity [J]. Journal of Inorganic Biochemistry, 1983,18:195-211
[17]  Haylock S J, Buckley P D, Blackwellel L F. The relationship of chromium to the glucose tolerance factorⅡ[J]. Journal of Inorganic Biochemistry, 1983,19 :105-117
[18]  黄仲贤.金属蛋白研究中几个值得注意的动向[J].化学进展, 2002,14(4):318-322

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