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核农学报  2012 

水稻DNA损伤修复同源基因纯合突变系的鉴定

, PP. 409-415

Keywords: 水稻,T-DNA,突变系,Tos17突变系,DNA损伤修复

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Abstract:

在长期的进化过程中,生物逐步形成了应对各种DNA损伤的修复机制,以保护基因组的完整性,减轻或消除DNA损伤的影响。本研究通过同源搜索找到了与DNA损伤修复相关水稻同源基因,在水稻插入突变体库中搜索出插入突变系,并成功引进了其中的26份T1代突变材料。通过对插入位点的分子鉴定和进一步培育,获得了14份涉及8个水稻DNA损伤修复同源基因的T3代纯合插入突变系,并对其进行了初步的遗传分析和表型考察。这些纯合突变系涉及的水稻基因可能在DNA错配修复(Os02g0592300、Os09g0407600、Os10g0509000)和碱基切除修复(Os02g0465112、Os06g0643600、Os05g0567500、Os04g0673400、Os09g0420300)中发挥重要作用。与亲本相比,除1份材料外,其他纯合突变体系及其野生型姐妹系的结实率均有显著降低;某些纯合突变系的千粒重与亲本相比有显著下降。这些水稻DNA损伤修复基因突变系的育成为开展水稻DNA修复的遗传学和分子生物学研究以及培育抗逆水稻提供了珍贵的实验材料。

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