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核农学报  2012 

食品辐照标志物DiHT的人工抗原合成及鉴定

, PP. 511-516

Keywords: 二氢脱氧胸腺嘧啶,半抗原,人工抗原,酶联免疫

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Abstract:

以二氢脱氧胸腺嘧啶(DiHT)为初始反应物,应用琥珀酸酐法合成二氢脱氧胸腺嘧啶的衍生物,即半抗原DiHT-COOH,经薄层色谱(TLC)和核磁共振波谱(NMR)对其进行鉴定;采用混合酸酐法与活化酯法分别将半抗原与载体蛋白BSA、OVA进行偶联,制备二氢脱氧胸腺嘧啶的人工抗原和包被原,通过紫外扫描、SDS-PAGE凝胶电泳方法对其进行鉴定,偶联比分别为9.81和121。人工抗原偶联成功后免疫BALB/c小鼠制备多抗血清,间接非竞争ELISA方法检测效价达12.56×104,表明人工抗原合成成功。

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