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核农学报  2014 

水稻不同Wx等位基因对籽粒直链淀粉积累特性的影响

DOI: 10.11869/j.issn.100-8551.2014.10.1760, PP. 1760-1764

Keywords: 水稻,Wx基因,等位基因,直链淀粉,积累特性

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Abstract:

为了解水稻Wx等位变异对籽粒直链淀粉积累特性的影响,本研究选用携带有5种不同Wx等位基因的水稻单片段代换系(Single-segmentsubstitutionline,SSSL)为材料,在同一遗传背景下对水稻籽粒灌浆过程中表观直链淀粉含量(Apparentamylosecontent,AAC)的动态变化及其与颗粒结合性淀粉合成酶(Granule-boundstarchsynthase,GBSS)酶活的关系进行了研究.结果表明,不同Wx等位基因水稻材料籽粒灌浆过程中的AAC差异在灌浆早期(花后6d)已较明显,随着籽粒发育,非糯水稻花后6~11d是直链淀粉的快速增长期.籽粒灌浆过程中,不同Wx等位基因间籽粒AAC存在显著性差异,并且在直链淀粉积累特性上存在明显不同.较高和高AAC品系(携Wxg2和Wxg3基因)直链淀粉积累速率快,在灌浆初期(花后11d)便达到与籽粒成熟期一致的水平,之后变化不大;低、中等AAC材料(携Wxt和Wxg1基因)直链淀粉积累速率相对中等,灌浆初期(花后11d)AAC并不高,其直链淀粉的积累是个渐近的过程,直至灌浆后期(花后26d)才达到最大含量;糯稻(携wx基因)则在整个籽粒发育过程中AAC变化不大,始终保持着极低的含量水平.在整个灌浆期间,籽粒GBSS酶活与AAC均呈正相关,其中灌浆早期(花后6d)相关度最高,随后相关度逐渐降低,但始终维持在较高的水平(r>0.820).本研究结果可为进一步深入研究Wx座位等位变异机制奠定基础.

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