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核农学报  2014 

黑水虻幼虫蛋白质的制备及体外抗氧化活性

DOI: 10.11869/j.issn.100-8551.2014.11.2001, PP. 2001-2009

Keywords: 黑水虻,幼虫,蛋白质提取,碱提,酸沉,体外抗氧化

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Abstract:

本文主要研究黑水虻幼虫蛋白质的制备及其体外抗氧化活性.以黑水虻幼虫为原料,采用碱提酸沉法提取黑水虻幼虫蛋白质,并于等电点沉淀,沉淀复溶后再浓缩、透析、冻干.分别测定了其总还原力,超氧阴离子自由基清除活性、羟基自由基清除活性、DPPH自由基清除活性、ABTS自由基清除活性、金属离子螯合能力及脂质体系中抗氧化能力,并将其体外抗氧化能力与维生素C(测定金属离子螯合能力时与EDTA)和卵清白蛋白进行比较.结果显示:BSFLP的等电点在4.8左右,并且该法所提蛋白纯度较高,表明利用碱提酸沉法对黑水虻幼虫进行蛋白的提取的方法可行.BSFLP的总还原力约是VC的0.013倍,是OVA的1.319倍;BSFLP的超氧阴离子自由基清除活性强于OVA,而弱于VC;其清除羟基自由基、清除DPPH自由基、清除ABTS自由基、螯合亚铁离子、亚油酸体系中抑制过氧化的IC50分别为2.577、3.965、0.228、0.876、5.050mg·mL-1,也均介于VC(或EDTA)和OVA之间,说明BSFLP具有较高的体外抗氧化活性.本文为黑水虻的进一步研究与综合利用及抗氧化功能蛋白食品的研发提供了一定的依据.

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