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百合根外植体体细胞胚胎再生影响因素研究

DOI: 10.11869/j.issn.100-8551.2015.08.1494, PP. 1494-1501

Keywords: 百合,根外植体,体细胞胚胎发生,N-1-萘基酞氨酸(NPA)

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Abstract:

为了探讨生长素对百合根外植体体细胞胚胎再生的影响,采用生长素运输抑制剂(NPA)对根外植体体胚诱导及发育进行研究,同时对不同根长外植体和不同根段外植体的体胚发生能力、不同激素对体胚扩增的影响展开研究。结果表明:培养基中添加NPA对根外植体的胚性愈伤组织诱导抑制作用明显,体细胞胚胎发育畸形。含有根尖的根处植体均能诱导产生胚性愈伤组织,诱导率均高于94.4%;对照(不含根尖的根外植体)及远离根尖的根段外植体诱导产生少量胚性愈伤组织,诱导率最高仅为8.9%。0.3cm根外植体(含根尖)生长系数最高,为36.8;单位重量根外植体(含根尖不同长度)产生的体胚数为21.8~26.3,差异不显著。在含有0.5mg·L-1Picloram的培养基中球形胚增殖率最高,达98.9%;在含有0.2mg·L-1Picloram的培养基中球形胚增殖系数最高,为20.1。结果表明内源生长素的含量及外源生长素的添加与百合根体细胞胚胎诱导关系密切,内源生长素运输参与百合体细胞胚胎的诱导及形态建成。本研究为百合根体细胞胚胎发生的分子机理研究奠定基础。

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