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二倍体与同源四倍体茅苍术基因组DNA甲基化水平与模式的MSAP分析

DOI: 10.11869/j.issn.100-8551.2015.08.1502, PP. 1502-1508

Keywords: 茅苍术,同源四倍体,表观遗传,基因组,甲基化敏感扩增多态性(MSAP)

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Abstract:

为探究二倍体和同源四倍体茅苍术表观遗传差异,以不同倍性的茅苍术为试验材料,应用MSAP技术研究其基因组DNA甲基化水平及模式变化。本试验选用20对选扩引物,在茅苍术二倍体和同源四倍体中检测到的基因位点数分别为520和527,其相应的扩增总甲基化率为56.92%和55.03%,四倍体基因组DNA半甲基化率高于二倍体,而全甲基化率低于二倍体;四倍体与二倍体相比,DNA甲基化模式发生调整的基因位点占总检测位点的52.53%。本研究为深入揭示四倍体茅苍术的表观遗传调控机制奠定了基础。

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