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核农学报  2014 

四倍体青花菜小孢子培养及胚胎发育途径研究

DOI: 10.11869/j.issn.100-8551.2014.08.1358, PP. 1358-1364

Keywords: 青花菜,四倍体,小孢子培养,发育途径

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Abstract:

以同源四倍体青花菜为试材,进行了游离小孢子培养的胚胎诱导条件及胚胎发育途径的研究。结果表明:基因型是影响四倍体青花菜小孢子胚胎诱导率的重要因子,基因型A36的出胚率最高,达到28.5个·蕾-1;热激胁迫(32.5℃)诱导小孢子从配子体发育途径向胚胎发生途径转换,其小孢子胚胎发育途径为典型的单核期小孢子经对称分裂产生胚胎的途径(B途径);4℃低温预处理1d或2d结合32.5℃热激1d后,对称分裂和多细胞团增加,供试基因型小孢子出胚率显著提高。本研究将有助于游离小孢子胚胎发生机理的研究,以及其他芸薹属小孢子胚胎体系的建立及优化。

References

[1]  Takahata Y, Keller W A. High frequency embryogenesis and plant regeneration in isolated microspore culture of Brassica oleracea L[J]. Plant Science, 1991, 74(2):235-242
[2]  Duijs J G, Voorrips R E, Visser D L, and Custers J B M. Microspore culture is successful in most crop types of Brassica oleracea L[J]. Euphytica, 1992, 60(1): 45-55
[3]  Dias J S. Effect of incubation temperature regimes and culture medium on broccoli microspore culture embryogenesis[J]. Euphytica, 2001, 119(3):389-394
[4]  冯翠, 曾爱松, 严继勇, 宋立晓, 高兵, 侯喜林. 影响青花菜游离小孢子培养的因素[J]. 南京农业大学学报, 2011, 34(6):20-24.
[5]  张振超, 耿鑫鑫, 戴忠良, 潘跃平, 王兵, 许玲, 颜志明, 周伟军. 甘蓝类植物小孢子培养及植株再生研究[J]. 核农学报, 2013, 27(7):929-937.
[6]  Hu T, Kasha KJ. A cytological study of pretreatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris[J]. Genome, 1999, 42(3):432-441
[7]  秦瑞珍, 宋文昌, 郭秀平.同源四倍体水稻花药培养在育种中的应用[J]. 中国农业科学, 1992, 25(1):6-13
[8]  申书兴, 陈苏, 李振秋, 张成台, 梁会芬. 四倍体大白菜小孢子培养获得初级三体的细胞学观察[J]. 园艺学报, 2000, 27(2):145-147
[9]  秦瑞珍, 程治军, 郭秀平. 利用同源四倍体花培途径创建水稻突变体群的研究[J]. 作物学报, 2005, 31(3):392-394
[10]  Seguí-Simarro J M, Corral-Martínez P, Parra-Vega V, González-García B. Androgenesis in recalcitrant solanaceous crops[J]. Plant Cell Reports, 2011, 30(5):765-778
[11]  袁素霞. 结球甘蓝和青花菜游离小孢子培养及早期胚胎形成相关基因的差异表达分析[D]. 北京:中国农业科学院, 2009
[12]  曾爱松, 冯翠, 高兵, 宋立晓, 严继勇. 结球甘蓝小孢子培养技术体系的优化研究[J]. 华北农学报, 2010, 25(增刊):40-44
[13]  宋立晓, 冯翠, 曾爱松, 高兵, 严继勇. 影响青花菜游离小孢子培养胚胎发生的因子[J]. 江苏农业学报, 2010, 26(6):1319-1322
[14]  Petricka J J, Van-Norman J M, Benfey P N. Symmetry breaking in plants: molecular mechanisms regulating asymmetric cell divisions in Arabidopsis[J]. Cold Spring Harbor Perspectives in Biology, 2009, 1(5):a000497
[15]  Jonzález-Melendi P, Testillano P S, Ahmadian P, Reyes J, Risueno M C. Immunoelectron microscopy of PCNA as an efficient marker for studying replication times and sites during pollen development[J]. Chromosoma, 2000, 109(6):397-409
[16]  Testillano P S, Georgiev S, Mogensen H L, Coronado M J, Dumas C, Risueno M C, Matthys-Rochon E. Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis[J]. Chromosoma, 2004, 112(7):342-349
[17]  González-Melendi P, Ramírez C, Testillano P, Kumlehn J, Risuen? M. Three dimensional confocal and electron microscopy imaging define the dynamics and mechanisms of diploidisation at early stages of barley microspore-derived embryogenesis[J]. Planta, 2005, 222(1):47-57
[18]  Oleszczuk S, Sowa S, Zimny J. Androgenic response to preculture stress in microspore cultures of barley[J]. Protoplasma, 2006, 228(1):95-100
[19]  Segui-Simarro J M, Testillano P S, Risueno M C. Hsp70 and Hsp90 change their expression and subcellular localization after microspore embryogenesis induction in Brassica napus L[J]. Journal of Structural Biology, 2003, 142(3):379-391
[20]  Bárány I, Fadón B, Risueňo MC, Testillano PS. Cell wall components and pectin esterification levels as markers of proliferation and differentiation events during pollen development and embryogenesis[J]. Journal of Experimental Botany, 2010, 61(4):1159-1175
[21]  曾爱松, 冯翠, 高兵, 宋立晓, 严继勇. 温度胁迫对结球甘蓝游离小孢子胚胎发生的影响[J].江苏农业学报. 2011, 27(3):623-627
[22]  Touraev A, Indrianto A, Wratschko I, Vicente O, Heberle-Bors E. Efficient microspore embryogenesis in wheat (Triticum aestivum L.) induced by starvation at high temperature[J]. Sexual Plant Reproduction, 1996, 9(4):209-215
[23]  Smykal P, Pechan P M. Stress, as assessed by the appearance of sHsp transcripts, is required but not sufficient to initiate androgenesis[J]. Physiologia Plantarum, 2000, 110(1):135-143
[24]  Gland A, Lichter R, Schweiger H G. Genetic and exogenous factors affecting embryogenesis in isolated microspore culture of Brassica napus L[J]. Journal of Plant Physiol, 1988, 132(5):613-617
[25]  Davies P A, Morton S. A comparison of barley isolated microspore and anther culture and the influence of cell culture density[J]. Plant Cell Reports, 1998, 17(3):206-210
[26]  Kasha K J, Simion E R, Yao Q A, Hu T C, Carlson A R. An improved in vitro technique for isolated microspore culture of barley[J]. Euphytica, 2001, 120(3):379-385
[27]  Indrianto A E, Bors H, Touraev A. Assessment of various stresses and carbohydrates for their effect on the induction of embryogenesis in isolated wheat microspores[J]. Plant Science, 1999, 143(1):71-79
[28]  Gaillard A P, Beckert M. Optimization of maize microspore isolation and conditions for reliable plant regeneration[J].Plant Cell Reports, 1991, 10(1):55-58
[29]  Yuan S X, Liu Y M, Fang Z Y, Yang L M, Zhuang M, Zhang Y Y, Sun P T. Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli[J]. Plant Breeding, 2011, 130(1):80-85
[30]  Dunwell J M, Cornish M, and Cource A G L. Influence of genotype, plant growth temperature and anther incubation temperature on microspore embryo production in Brassica napus ssp. oleifera[J]. Journal of Experimental Botany, 1985, 36(4):679-689
[31]  Mohan Janin S, Sopory S K, Veilleux R E. In Vitro Haploid Production in Higher Plants: Applications Volume 2[M]. Netherlands: Springer, 1996

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