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核农学报  2014 

高压脉冲电场对大肠杆菌噬菌体灭活效果初探

DOI: 10.11869/j.issn.100-8551.2014.08.1440, PP. 1440-1445

Keywords: 大肠杆菌,高压脉冲电场,灭活,噬菌体

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Abstract:

为探讨高压脉冲电场(pulsedelectricfield,PEF)在噬菌体污染治理方面的工业应用,研究了高压脉冲电场对λ噬菌体、ΦX174噬菌体以及宿主菌E.coliMV1184的灭活效果。采用不同电场强度E,脉冲个数N和脉冲宽度г体系对λ噬菌体、ΦX174噬菌体以及λ噬菌体和E.coliMV1184混合悬液进行处理。结果表明在输出电压1~10kV,脉冲宽度1~10μs范围内,灭活效率随着脉冲数的增加而提高。PEF对噬菌体的灭活效率并不依赖于噬菌体的颗粒结构,但噬菌体颗粒结构对脉冲宽度г灭活效率有影响。在噬菌体与宿主菌E.coliMV1184的混合悬液中,λ噬菌体对PEF的灭活作用更加敏感,失活率显著高于E.coliMV1184。高压脉冲电场可以用于常温下溶液中噬菌体的灭活处理并达到消毒水平,是一种高效的噬菌体灭活方法。

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