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核农学报  2014 

不同氮水平对原产地和入侵地飞机草(ChromolaenaodorataL.)DNA甲基化的影响

DOI: 10.11869/j.issn.100-8551.2014.09.1591, PP. 1591-1596

Keywords: 飞机草,DNA甲基化,氮水平,MSAP

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Abstract:

为探究原产地和入侵地飞机草DNA甲基化多态性的变化和氮营养水平对飞机草DNA甲基化多态性的影响,本文用水培法分别培养来自原产地墨西哥和入侵地西双版纳的飞机草(ChromolaenaodorataL.),对两地飞机草进行了高氮(315mg·L-1)、正常(中)氮(210mg·L-1)和低氮(105mg·L-1)3种氮水平处理,采用MSAP(甲基化敏感扩增多态性)技术测定试材的DNA甲基化水平。结果表明,无论是原产地还是入侵地的飞机草,在高氮和低氮处理条件下均发生了DNA甲基化水平和模式的改变;且入侵地飞机草DNA甲基化水平高于原产地飞机草,飞机草DNA甲基化水平都随着氮供应水平的升高而升高。本研究为从表观遗传角度揭示外来植物的入侵机制提供依据。

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