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核农学报  2014 

大豆自然群体SSR标记遗传多样性及其与农艺性状的关联分析

DOI: 10.11869/j.issn.100-8551.2014.09.1576, PP. 1576-1584

Keywords: 大豆,SSR,遗传多样性,群体结构,关联分析,等位变异

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Abstract:

分析大豆亲本材料的遗传多样性,发掘农艺性状关联位点的优异等位变异,为大豆杂交组合的配置及分子标记辅助育种提供依据。本试验利用59个SSR标记对90份大豆种质资源进行多态性扫描和遗传多样性分析。筛选出46个标记,应用STRUCTURE2.3.2软件进行群体结构遗传分析,利用Tassel2.1软件MLM模型对18个农艺性状进行关联分析,发掘优异等位变异。结果表明:(1)59个标记中50个标记具有多态性,共检测出154个等位变异;多态性信息值PIC分布范围为0.042~0.765,平均PIC=0.421;SSR标记位点的Shannon指数(H’)的分布范围为0.1066~1.6804,平均值为0.8241;遗传距离的变异范围为0.0307~1.4529,平均值0.7015。(2)供试材料分为4个亚群。发现7个与分枝数、百粒重、叶形、主茎节数、生育期和蛋白含量相关联的标记,表型变异的解释率为0.003~0.339。研究在各关联位点找到了Satt263-A279、Satt156-A203、Satt567-A112等表型效应明显的优异等位变异。为大豆育种优异基因的克隆提供科学依据。

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