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核农学报  2014 

油菜LEAFYCOTYLEDON2(BnLEC2)基因的克隆及表达分析

DOI: 10.11869/j.issn.100-8551.2014.12.2175, PP. 2175-2183

Keywords: 甘蓝型油菜,BnLEC2基因,基因克隆,进化分析,转录分析

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Abstract:

LEAFYCOTYLEDON2(LEC2)为具有B3结构域的DNA结合蛋白家族的成员,是调控胚胎形成、种子储藏蛋白代谢和脂肪酸代谢的重要转录因子。本文对四倍体甘蓝型油菜BnLEC2基因进行了克隆,并分析了白菜BrLEC2、甘蓝BoLEC2和甘蓝型油菜BnLEC2同源基因的进化及其表达模式。结果表明,油菜、白菜和甘蓝LEC2同源基因在进化上较为保守;但部分白菜和甘蓝LEC2同源基因的表达发生沉默。BnLEC2同源基因表达模式与其对应的白菜和甘蓝LEC2发生明显改变,前者主要在种子发育的中期高水平表达,这与油菜种子油脂等储藏物质的积累期重叠。表明BnLEC2同源基因通过改变其表达模式影响油菜种子储存物质的积累。这些结果初步揭示了多拷贝BnLEC2对油菜种子储存物质调控的协同表达模式。

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