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核农学报  2015 

平榛MAPKK基因克隆及在非生物胁迫下的表达分析

DOI: 10.11869/j.issn.100-8551.2015.01.0049, PP. 49-55

Keywords: 平榛,MAPKK,基因克隆,非生物胁迫,表达模式

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Abstract:

通过研究外界刺激信号对平榛中促分裂原活化蛋白酶途径的影响,探索该途径中MAPKK类基因对各种非生物胁迫的响应机制。根据平榛花芽转录本高通量测序的结果,采用RACE-PCR方法克隆到一个平榛MAPKK基因,全长1065bp,推断其编码354个氨基酸,命名为ChMAPKK2。序列分析表明,该基因含有MAPKK类型基因典型的磷酸一致化序列SLADIDS。构建的系统发育树结果表明,它与葡萄VvMAPKK2以及苹果MdMAPKK的亲缘关系较近,相似性分别为81.69%和78.53%。采用qRT-PCR,以ACTIN为内参对ChMAPKK2基因在自然条件下花芽部位的表达模式进行分析,结果表明:在自然条件下从11月份到次年4月份ChMAPKK2的表达量呈现先上升后下降的趋势;对平榛根蘖苗进行4℃、干旱及盐胁迫处理,发现ChMAPKK2基因均受上述3种非生物胁迫的诱导而上调表达;ChMAPKK2在不同器官中的表达具有组织表达特异性,雌花芽中表达量最高,其次是树皮,雄花序中表达量最低。上述研究结果表明ChMAPKK2基因在平榛适应非生物胁迫的过程中发挥着重要作用。

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