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核农学报 2015

雨生红球藻诱变后生长速率和超微结构的变化

DOI: 10.11869/j.issn.100-8551.2015.04.0623, PP. 623-628

沈国明,牛健康,陆开形,尚宏芹

Keywords: 生长率,雨生红球藻,致死率,亚硝基胍,色素含量,超微结构

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Abstract:

为探讨亚硝基胍(N-methyl-N'-Nitro-N-Nitrosoguanidine,NTG)对雨生红球藻(Haematococcuspluvialis)生长速率及超微结构变化的影响,用不同浓度的NTG处理藻类,对藻类的生长速率、色素含量和超微结构的变化进行了研究.结果表明:即使低浓度(0.5g·L-1)的NTG对雨生红球藻也是致死的,致死率与NTG浓度呈正相关.藻类经中等浓度(2.5g·L-1)NTG诱变后,存活藻类的增长速率K值和虾青素含量分别达到0.381±0.0289和2.9±0.29mg·L-1.低浓度NTG处理藻类其叶绿素含量略有上升,但高浓度(3.5g·L-1)时叶绿素含量明显减少.低NTG浓度处理时藻类细胞结构变化不大,但高浓度处理时细胞膜、线粒体及叶绿体等均遭受不同程度的损伤.本研究可为深入理解NTG诱变藻类的生理生化机制提供借鉴.

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