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核农学报  2015 

基于数字基因表达谱筛选黄瓜中水杨酸诱导基因

DOI: 10.11869/j.issn.100-8551.2015.05.0874, PP. 874-884

Keywords: 黄瓜,水杨酸,数字基因表达谱,高通量测序,差异表达基因,系统获得性抗性

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Abstract:

本文通过筛选水杨酸诱导黄瓜叶片差异表达基因(DEGs),旨在揭示水杨酸提高黄瓜抗病性机制和信号转导过程.以抗霜霉病黄瓜东农649为试材,无菌培养幼苗至4叶期,喷施5mmol·L-1的水杨酸,喷施前和喷施后3、12和24h分别采取叶片,提取总RNA,制备cDNA文库,应用Illumina高通量测序技术对水杨酸处理前后的4个叶片cDNA文库进行差异基因数字表达谱分析.结果表明,SA诱导了多数与叶绿体和细胞壁相关基因的下调表达和多个与抗病相关基因的上调表达,对受体激酶、细胞色素P450、脂氧合酶和脂转运蛋白等信号转导相关基因的表达均有显著影响.本研究获取了丰富有效的数据,初步筛选的DGEs涉及到植物的PCD(程序性细胞死亡)、信号转导、系统获得性抗性(SAR)形成等过程,证实了SA与上述过程有密切关系,为最终揭示SA提高黄瓜抗病性机制奠定了基础.

References

[1]  Park S W, Liu P P, Forouhar F, Vlot A C, Tong L, Tietjen K, Klessig D F. Use of a synthetic salicylic acid analog to investigate the roles of methyl salicylate and its esterases in plant disease resistance[J]. The Journal of Biological Chemistry, 2009, 284(11):7307-7317
[2]  Vlot A C, Dempsey D A, Klessig D F. Salicylic acid, a multifaceted hormone to combat disease[J]. Annual Review of Phytopathology, 2009,47:177-206
[3]  孙艳,杨淑英.水杨酸诱发黄瓜幼苗对霜霉病抗性的研究[J].植物病理学报, 2004,34(2): 187-189
[4]  An C, Mou Z. Salicylic acid and its function in plant immunity[J]. Journal of Integrative Plant Biology, 2011, 53(6):412-428
[5]  Klessig D F, Durner J, Noad R, Navarre D A, Wendehenne D, Kumar D, Zhou J M, Shah J, Zhang S, Kachroo P, Trifa Y, Pontier D, Lam E, Silva H. Nitric oxide and salicylic acid signaling in plant defense[J]. Proceedings of the National Academy of Sciences, 2000, 97(16):8849-8855
[6]  李兆亮,原永兵,刘连成,曹宗巽.黄瓜细胞中水杨酸的信号传递研究[J].植物学报, 1998,40(5):430-436
[7]  Blanco F, Garreton V, Frey N, Dominguez C, Pérez-Acle T, Van der Straeten D, Jordana X, Holuigue L. Identification of NPR1-dependent and independent genes early induced by salicylic acid treatment in Arabidopsis[J]. Plant Molecular Biology, 2005, 59(6):927-944
[8]  Chen J, Zhu C, Li L P, Sun Z Y, Pan X B. Effects of exogenous salicylic acid on growth and H2O2-metabolizing enzymes in rice seedlings under lead stress[J]. Journal of Environmental Sciences, 2007, 19(1):44-49
[9]  Zhang W P, Jiang B, Lou L N, Lu M H, Yang M, Chen J F. Impact of salicylic acid on the antioxidant enzyme system and hydrogen peroxide production in Cucumis sativus under chilling stress[J]. Zeitschrift Fur Naturforschung C-A Journal of Biosciences, 2011, 66(7/8):413-422
[10]  Sadumpati V, Kalambur M, Vudem D R, Kirti P B, Khareedu V R. Transgenic indica rice lines, expressing Brassica juncea Nonexpressor of pathogenesis-related genes 1 (BjNPR1), exhibit enhanced resistance to major pathogens[J]. Journal of Biotechnology, 2013, 166(3):114-121
[11]  Chen X K, Zhang J Y, Zhang Z, Du X L, Du B B, Qu S C. Overexpressing MhNPR1 in transgenic Fuji apples enhances resistance to apple powdery mildew[J]. Molecular Biology Reports, 2012, 39(8):8083-8089
[12]  Wally O, Jayaraj J, Punja Z K. Broad-spectrum disease resistance to necrotrophic and biotrophic pathogens in transgenic carrots (Daucus carota L.) expressing an Arabidopsis NPR1 gene[J]. Planta, 2009, 231(1):131-141
[13]  Moreau M, Tian M, Klessig D F. Salicylic acid binds NPR3 and NPR4 to regulate NPR1-dependent defense responses[J]. Cell Research, 2012, 22(12):1631-1633
[14]  Fu Z Q, Yan S, Saleh A, Wang W, Ruble J, Oka N, Mohan R, Spoel S H, Tada Y, Zheng N, Dong X. NPR3 and NPR4 are receptors for the immune signal salicylic acid in plants[J]. Nature, 2012, 486(7402):228-232
[15]  Ee S F, Oh J M, Mohd Noor N, Kwon T R, Mohamed-Hussein Z A, Ismail I, Zainal Z. Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus[J]. Molecular Biology Reports, 2013, 40(3):2231-2241
[16]  Mahdavian K, Kalantari K M, Ghorbanli M, Torkzade M. The effects of salicylic acid on pigment contents in ultraviolet radiation stressed pepper plants[J]. Biologia Plantarum, 2008, 52(1):170-172
[17]  Khan W, Prithiviraj B, Smith D L. Photosynthetic responses of corn and soybean to foliar application of salicylates[J]. Journal Plant Physiololgy, 2003, 160(5):485-492
[18]  Kovácik J, Grúz J, Backor M, Strnad M, Repcák M. Salicylic acid-induced changes to growth and phenolic metabolism in Matricaria chamomilla plants[J]. Plant Cell Reports, 2009, 28(1):135-143
[19]  Yun L J, Chen W L. SA and ROS are involved in methyl salicylate-induced programmed cell death in Arabidopsis thaliana[J]. Plant Cell Reports, 2011, 30(7): 1231-1239
[20]  Nersissian A M, Immoos C, Hill M G, Hart P J, Williams G, Herrmann R G, Valentine J S. Uclacyanins, stellacyanins, and plantacyanins are distinct subfamilies of phytocyanins: plant-specific mononuclear blue copper proteins[J]. Protein Science, 1998, 7(9):1915-1929
[21]  田琴,李艳军,郭芳,张新宇,王海云,孙杰.棉纤维特异表达蓝铜蛋白基因(GhBCP1)的克隆与鉴定[J].中国农业科学, 2010, 43(3):612-617
[22]  梅伏生,孟祥高,赵显珍,李骥,廖展如,杨光富. 脂氧合酶—植物抗胁迫响应的关键酶[J].华中师范大学学报:自然科学版, 2005, 39(3):346-350, 354
[23]  刘新,张蜀秋.在伤信号传导中茉莉酸与水杨酸的关系[J].植物学通报, 2000, 17(2):133-136
[24]  Jung H W, Kim K D, Hwang B K. Identification of pathogen-responsive regions in the promoter of a pepper lipid transfer protein gene (CALTPI) and the enhanced resistance of the CALTPI transgenic Arabidopsis against pathogen and environmental stresses[J]. Planta, 2005, 221(3): 361-373
[25]  Sarowar S, Kim Y J, Kim K D, Hwang B K, Ok S H, Shin J S. Overexpression of lipid transfer protein(LTP)genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco[J]. Plant Cell Reports, 2009, 28(3): 419-427
[26]  Park C J, Shin R, Park J M, Lee G J, You J S, Paek K H. Induction of pepper cDNA encoding a lipid transfer protein during the resistance response to tobacco mosaic virus[J]. Plant Molecular Biology, 2002, 48(3):243-254
[27]  Loake G, Grant M. Salicylic acid in plant defence—the players and protagonists[J]. Current Opinion in Plant Biology, 2007, 10(5):466-472

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