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核农学报  2015 

桃已知MADS-box转录因子的生物信息学及花发育表达分析

DOI: 10.11869/j.issn.100-8551.2015.05.0849, PP. 849-858

Keywords: ,MADS-box,基因组学,生物信息学分析,表达分析

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Abstract:

MADS-box基因家族是研究最广泛的植物转录因子之一,参与调控植物生长和发育的多个过程.本文列举了当前已知的MADS-box(PpMADS)基因在桃(Prunuspersica)功能基因组数据库中的基本信息,对PpMADS基因的保守结构域、进化关系、内含子与外显子、染色体定位和保守元件进行了详细分析;并利用半定量RT-PCR技术分析了其在不同花器官中和的花的不同发育阶段表达水平.结果表明,PpMADS在桃不同花器官、花不同发育期中起重要的调控作用,为进一步筛选PpMADS基因进行功能分析奠定相关理论基础.

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