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核农学报  2015 

俄罗斯小麦籽粒硬度及puroindoline基因等位变异的分布

DOI: 10.11869/j.issn.100-8551.2015.05.0836, PP. 836-842

Keywords: 俄罗斯小计麦,籽粒硬度,Pina,Pinb,STS标记,TS-PCR

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Abstract:

小麦籽粒硬度是重要的品质性状之一,对小麦加工品质具有重要影响.为了合理引进种质资源,培育高品质小麦品种,本研究利用小麦硬度指数测定仪JYDB100×40对引进俄罗斯小麦品种的籽粒硬度进行检测,利用STS标记和变温PCR对控制籽粒硬度基因puroindoline的主要等位变异进行分析.结果显示:208个俄罗斯引进小麦品种硬度指数范围为45.6~79.2,硬质麦品种为190个(91.35%),混合麦品种为18个(8.65%).Pina基因有Pina-D1a、Pina-D1b和杂合Pina-D1a/Pina-D1b共3种类型,所占比例分别为87.02%、10.10%和2.88%.Pinb基因有Pinb-D1a、Pina-D1b、Pinb-D1c和双位点突变Pinb-D1bc4种类型,所占比例分别为37.02%、55.77%、3.85%和3.37%.统计结果显示:携带Pina-D1b+Pinb-D1a基因小麦籽粒硬度指数显著大于Pina-D1a+Pinb-D1b,Pina-D1a+Pinb-D1a和Pina-D1a+Pinb-D1bc.其他puroindoline等位变异之间没有显著差异.俄罗斯硬质麦种质资源丰富,是改善我国小麦籽粒硬度和品质的重要遗传材料.

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