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核农学报  2015 

褪黑素对秦艽原生质体抗UV-B环境胁迫的作用

DOI: 10.11869/j.issn.100-8551.2015.05.0830, PP. 830-835

Keywords: 褪黑素,单细胞凝胶电泳,原生质体,秦艽,UV-B

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Abstract:

为探讨褪黑素对UV-B辐射后的秦艽原生质体的影响,本研究采用单细胞凝胶电泳技术检测秦艽原生质体DNA损伤程度,选取尾部DNA含量(TailDNA%)作为DNA损伤程度的分析指标.MEL以0、1、10μmol·L-1的终浓度加入原生质体悬液中,UV-B辐射处理的辐照度为0.5W·m-2.试验分2组:1)原生质体分别用UV-B辐射处理不同的时间(0、5、10、30、60、120s),暗培养60min后电泳.2)原生质体被UV-B照射40s后,分别暗培养不同时间(0、1、2、3、4、6h),再电泳.结果显示在5~30s的时间内,辐射时间越长,TailDNA%越高,相同的辐射时间下,MEL浓度越高,TailDNA%越低;在1~6h培养时间内,MEL处理的样品,TailDNA%值均低于阳性对照组,并且TailDNA%值的大小与所处理的MEL的浓度呈反比关系.由此可见褪黑素降低了UV-B辐射诱导的植物原生质体DNA损伤程度,促进DNA损伤的修复.本试验应用SCGE技术,进一步揭示了MEL在植物抗环境胁迫中所起到的促进作用.

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