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核农学报  2015 

2,4-表油菜素内酯对低温胁迫下辣椒幼苗根系生长及抗氧化酶系统的影响

DOI: 10.11869/j.issn.100-8551.2015.05.1001, PP. 1001-1008

Keywords: 2,4-表油菜素内酯,低温,辣椒幼苗,根系形态,抗氧化酶

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Abstract:

为探讨外源2,4-表油菜素内酯(2,4-epibrassinolide,EBR)对低温胁迫下辣椒(CapsicumannuumL.)幼苗根系的生长和根系抗氧化系统的影响,本试验采用叶面喷施外源EBR(0、10.0、1.0、0.1、0.01、0.001μmol·L-1)的方法,比较分析了低温胁迫下幼苗根系形态、根系抗氧化酶活性、过氧化物质含量等生理指标.结果表明,低温抑制了辣椒幼苗根系的生长,低温胁迫下喷施EBR,辣椒幼苗根系的生长受抑制程度减轻,其根系总长、根表面积、分根数较单纯低温处理显著增加;低温胁迫下喷施0.1μmol·L-1EBR辣椒幼苗根系SOD、POD、CAT活性显著提高,MDA含量降低.外源EBR处理可调节辣椒根系形态和提高根系抗氧化酶活性,增强植株抗低温胁迫的能力,并且EBR的浓度以0.1μmol·L-1最佳.说明在低温胁迫下适宜浓度的EBR可缓解辣椒幼苗的根系生长,这对阐明EBR缓解辣椒幼苗低温胁迫的作用机制提供了理论依据.

References

[1]  眭晓蕾, 毛胜利, 王立浩, 李伟, 张宝玺, 张振贤. 低温对弱光影响甜椒光合作用的胁迫效应[J]. 核农学报, 2008, 22(6):880-886
[2]  孙三杰, 李建明, 宗建伟, 姚勇哲, 陈凯利. 亚低温与干旱胁迫对番茄幼苗根系形态及叶片结构的影响[J]. 应用生态学报, 2012, 23(11): 3027-3032
[3]  Xia X J, Wang Y J, Zhou Y H, Tao Y, Mao W H, Shi K, Asami T, Chen Z, Yu J Q. Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber [J]. Plant Physiology, 2009, 150(7): 801-814
[4]  Krishna P. Brassinosteroid-mediated stress responses [J]. Journal of Plant Growth Regulation, 2003, 22(4): 289-297
[5]  Haubrick L L, Assmann S M. Brassinosteroids and plant function: some clues, more puzzles [J]. Plant, Cell and Environment, 2006, 29:446-457
[6]  Hu W H, Yan X H, Xiao Y A, Zeng J J, Qi H J, Ogweno J O. 24-Epibrassinosteroid alleviate drought-induced inhibition of photosynthesis in Capsicum annuum [J]. Scientia Horticulturae, 2013, 150(2):232-237
[7]  Fariduddin Q, Khalil R R, Mir B A, Yusuf M, Ahmad A. 24-Epibrassinolide regulates photosynthesis, antioxidant enzyme activities and proline content of Cucumis sativus under salt and/or copper stress [J]. Environmental Monitoring and Assessment, 2013, 185(9):7845-7856
[8]  Talaat N B, Shawky B T. 24-Epibrassinolide alleviates salt-induced inhibition of productivity by increasing nutrients and compatible solutes accumulation and enhancing antioxidant system in wheat (Triticum aestivum L.) [J]. Acta Physiologiae Plantarum, 2013, 35(3): 729-740
[9]  张红. 硝普钠、24-表油菜素内酯\\水杨酸浸种对盐胁迫下玉米种子萌发及幼苗生长的影响[J]. 核农学报, 2012, 26(6):164-169
[10]  Farooq M, Wahid A, Lee D J, Cheema S A, Aziz T. DROUGHT STRESS: Comparative time course action of the foliar applied glycinebetaine, salicylic acid, nitrous oxide, brassinosteroids and spermine in improving drought resistance of rice [J]. Journal of Agronomy and Crop Science, 2010, 196(5): 336-345
[11]  Jiang Y P, Huang L F, Cheng F, Zhou Y H, Xia X J, Mao W H, Yu J Q. Brassinosteroids accelerate recovery of photosynthetic apparatus from cold stress by balancing the electron partitioning, carboxylation and redox homeostasis in cucumber[J]. Physiologia plantarum, 2013, 148(1): 133-145
[12]  Fariduddin Q, Yusuf M, Chalkoo S, Hayat S, Ahmad A. 28-homobrassinolide improves growth and photosynthesis in Cucumis sativus L. through an enhanced antioxidant system in the presence of chilling stress[J]. Photosynthetica, 2011, 49(1): 55-64
[13]  Kang Y Y, Guo S R, Li J, Duan J J. Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia [J]. Plant Growth Regulation, 2009, 57(3): 259-269
[14]  Yusuf M, Fariduddin Q, Ahmad A. 24-Epibrassinolide modulates growth, nodulation, antioxidant system, and osmolyte in tolerant and sensitive varieties of Vigna radiata under different levels of nickel: A shotgun approach [J]. Plant Physiology and Biochemistry, 2012, 57(8): 143-153
[15]  Hasan S A, Hayat S, Ahmad A. Brassinosteroids protect photosynthetic machinery against the cadmium induced oxidative stress in two tomato cultivars [J]. Chemosphere, 2011, 84(10): 1446-1451
[16]  Sharma I, Bhardwaj R, Pati P K. Stress modulation response of 24-epibrassinolide against imidacloprid in an elite indica rice variety Pusa Basmati-1[J]. Pesticide Biochemistry and Physiology, 2013, 105(2): 144-153
[17]  Li L, Xu J, Xu Z H, Xue H W. Brassinoeroids stimulate plant tropisms through modulation of polar auxin transport in Brassica and Arabidopsis [J]. Plant Cell, 2005, 17(10):2738-2753
[18]  Goetz M, Godt D E, Roitsch T. Tissue-specific induction of the mRNA for an extracellular invertase isoenzyme of tomato by brassinosteroids suggests a role for steroid hormones in assimilate partitioning [J]. The Plant Journal, 2000, 22(6): 515-522
[19]  Hayat S, Ali B, Hasan S A, Ahmad A. Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea [J]. Environmental and Experimental Botany, 2007, 60(1):33-41
[20]  Ding H D, Zhu X H, Zhu Z W, Yang S J, Zha D S, Wu X X. Amelioration of salt-induced oxidative stress in eggplant by application of 24-epibrassinolide [J]. Biologia Plantarum, 2012, 56(4): 767-770
[21]  Rady M M. Effect of 24-epibrassinolide on growth, yield, antioxidant system and cadmium content of bean (Phaseolus vulgaris L.) plants under salinity and cadmium stress [J]. Scientia Horticulturae, 2011, 129(2): 232-237
[22]  Ma Y H, Guo S R. 24-epibrassinolide improves cucumber photosynthesis under hypoxia by increasing CO2 assimilation and photosystem II efficiency [J]. Photosynthetica, 2014, 52(1): 96-104
[23]  李合生. 植物生理生化实验原理与技术[M]. 北京: 高等教育出版社, 2001
[24]  Giannopolitis C N, Ries S K. Superoxide dismutase I. Occurrence in higher plants [J]. Plant Physiology, 1977, 59(2): 309-314
[25]  Kochba J, Lavee S, Spieggl-Roy P. Differences in peroxidase activity and isoenzymes inembryogenic and non-embryogenic ‘Shamouti’ orange ovular callus lines[J]. Plant and Cell Physiology, 1977, 18(2):463-467
[26]  Aebi H. Catalase in vitro [J]. Methods in Enzymology, 1984, 105(13):121-126
[27]  田生科, 李廷轩, 彭红云, 杨肖娥, 李廷强, Ejaz. 铜胁迫对海州香薷和紫花香薷根系形态及铜富集的影响[J]. 水土保持学报, 2005, 19(3):97-100
[28]  Pierret A, Doussan C, Capowiez Y, Bastardie F, Pages L.Root functional architecture: A framework for modeling the inter-play between roots and soil [J]. Vadose Zone Journal, 2007, 6(2): 269-281
[29]  Hu W H, Wu Y, Zeng J Z, He L, Zeng Q M. Chill-induced inhibition of photosynthesis was alleviated by 24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery [J]. Photosynthetica, 2010, 48 (4): 537-544
[30]  康云艳, 杨暹, 郭世荣, 张营营. 24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响[J]. 中国农业科学, 2011, 44(12):2495-2503
[31]  Müssig C, Shin G H, Altmann T. Brassinosteroids promote root growth in Arabidopsis [J]. Plant Physiology, 2003, 133(3): 1261-1271.
[32]  康云艳, 郭世荣, 李娟, 段九菊. 24-表油菜素内酯对低氧胁迫下黄瓜幼苗根系抗氧化系统的影响[J]. 中国农业科学, 2008,41(1):153-161
[33]  Liu Y J, Zhao Z G, Si J, Di C X, Han J, An L Z. Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana [J]. Plant Growth Regulation, 2009, 59(3): 207-214
[34]  Choudhary S P, Kanwar M, Bhardwaj R, Gupta B D, Gupta R K. Epibrassinolide ameliorates Cr (VI) stress via influencing the levels of indole-3-acetic acid, abscisic acid, polyamines and antioxidant system of radish seedlings [J]. Chemosphere, 2011, 84(5): 592-600
[35]  惠竹梅, 王智真,胡勇, 邓敏敏, 张振文. 24-表油菜素内酯对低温胁迫下葡萄幼苗抗氧化系统及渗透调节物质的影响[J]. 中国农业科学, 2013, 46(5):1005-1013
[36]  肖春燕, 邢潇晨, 刘会芳, 徐巍, 崔金霞. 低温下NO对黄瓜光合荧光及抗氧化特性的影响[J]. 核农学报, 2014, 28(6):1083-1091
[37]  Kang Y Y, Guo S R, Duan J J, Hu X H. Effects of 24-epibrassinolide on antioxidant system and anaerobic respiratory enzyme activities in cucumber roots under hypoxia stress [J]. Journal of Plant Physiology and Molecular Biology, 2006, 32(5): 535-542
[38]  尚庆茂, 宋士清, 张志刚, 郭世荣. 外源BR诱导黄瓜(Cucumis sativus L.)幼苗的抗盐性[J]. 中国农业科学, 2006,39(9):1872-1877
[39]  Jager C E, Symons G M, Ross J J, Reid J B. Do brassinosteroids mediate the water stressresPonse? [J] Physiologia Plantarum, 2008, 133(2): 417-425
[40]  Cao S Q, Xu Q T, Cao Y J, Qian K, An K, Zhu Y, Hu B Z, Zhao H F, Kuai B K. Loss-of-function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis [J]. Physiologia Plantarum, 2005, 123(1):57-66

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