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核农学报  2015 

山羊妊娠期日粮添加酵母硒对后代羔羊睾丸GPxs表达的影响

DOI: 10.11869/j.issn.100-8551.2015.05.0859, PP. 859-865

Keywords: 母山羊,妊娠期,睾丸,Gpxs,mRNA表达,酵母硒

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Abstract:

为探明妊娠母山羊日粮中酵母硒水平对其所产后代公羔睾丸GPxs活性、睾丸组织GPx1,GPx3和GPx4mRNA表达及其GPx4蛋白表达的影响,采用实时定量PCR进行睾丸GPxsmRNA定量分析,并采用免疫荧光技术对GPx4蛋白定量分析.结果显示妊娠母山羊日粮中添加1.0mg·kg-1硒组(II组)可极显著提高后代公羔睾丸GPxs的活性(P<0.01).与对照组相比,II组GPx1mRNA表达量提高了0.84倍,GPx3mRNA表达量提高了2.6倍,GPx4mRNA表达量提高5倍,GPx4蛋白表达量显著增加(P<0.01),但4.0mg·kg-1硒组后代睾丸GPx4蛋白有过表达现象.妊娠母羊日粮添加1.0mg·kg-1酵母硒(日粮硒含量1.045mg·kg-1)可明显提高后代公羔睾丸中GPxs基因的表达.本研究不仅丰富了硒营养调控理论,而且对肉羊生产具有指导意义.

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