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核农学报  1996 

INDUCTIONOFSOMATICEMBRYOSANDREGENERATIONOFPLANTLETSFROMRICEROOTS

, PP. 216-220

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Abstract:

Thenumberofsomaticembryosandregeneratedplantletsweredirectlyinducedfromirra-diatedricerootsofthevarietyTaipei309(OryzasativaL.).Fortheinductionofsomaticem-bryos,thesuitableageofrootswasnomorethan7daysafterseedcultureonthemediumandthesectionsneartipweremorevigorousthanthosenearbaseofaroot;thenumberofsomaticem-bryosinducedbythemediawith1.11mg/L2,4-Dplus0.47mg/LNAAand2.21mg/L2,4-Dplus1.86mg/LNAAwasmuchhigherthanthatinducedby1.11mg/L2,4-Dalone;10~20Gyand40Gyofγirradiationwerethemostfavourableforincreasingsomaticembryonumberonthemediaof2.21mg/L2,4-Dplus1.86mg/LNAAand1.11mg/L2,4-Dplus0.47mg/LNAAor1.11mg/L2,4-Dalonerespectively.Thehighestnumberofregeneratedplantletswasfromthatculturedonthemediumof1.11mg/L2,4-Dplus0.47mg/LNAAandirradiatedwith30~40Gyofγrays.

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