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核农学报  2012 

不同磁场环境和培养基对拟南芥种子诱导愈伤组织的影响

, PP. 723-728

Keywords: 亚磁场,中强磁场,培养基,激素,愈伤组织诱导,拟南芥

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Abstract:

为探明磁场强度和培养基成份在拟南芥愈伤组织诱导中的作用,以野生型拟南芥(Columbia型)种子为材料,研究3种磁场环境(亚磁场、地磁场和中强磁场)、4种诱导培养基(NB1、NB2、MS1、MS2)条件下愈伤组织的诱导情况。结果显示,与地磁场相比,亚磁场对接种后第3~6天愈伤组织的发生有明显的抑制作用,中强磁场有明显的促进作用;在接种后第3~7天,NB2、MS2比NB1、MS1培养基促进了愈伤组织形成,且差异显著。愈伤组织净重反映了细胞增殖的状态,统计结果显示,与地磁场相比,亚磁场对细胞增殖没有明显影响,而中强磁场有显著的抑制作用;NB2、MS2比NB1、MS1培养基促进了细胞增殖,且差异显著。以上试验结果说明,磁场强度和培养基成份的变化对拟南芥种子诱导愈伤组织过程中第3~7天的愈伤组织形成和细胞增殖有不同程度影响,但2因素不存在交互作用,且2种因素对15d后的愈伤组织总出愈率无显著影响。6-BA对早期的愈伤组织诱导和细胞增殖均有促进作用,而且在地磁和亚磁场中,添加有6-BA的NB2和MS2培养基所诱导的愈伤组织色泽鲜亮,致密度适中,利于后续继代培养。

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