全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2012 

茶饮料主要组分及pH对HS-SPME萃取有机磷农药效率的影响

, PP. 911-918

Keywords: 有机磷农药,茶饮料,提取效率,茶多酚,蔗糖

Full-Text   Cite this paper   Add to My Lib

Abstract:

分析了茶饮料中2种主要组分蔗糖和茶多酚对顶空固相微萃取(HS-SPME)法萃取有机磷农药效率的影响,并根据这些结果建立了混合基质校准标准曲线。结果表明,蔗糖浓度在0~90g/L范围内明显降低5种农药(甲基对硫磷,马拉硫磷,毒死蜱,喹硫磷和乙硫磷)的提取回收率,几种农药之间差异不大。茶多酚浓度在200~1200mg/L范围内对5种农药的提取效率有明显的降低效应;在700和900mg/L时,各农药的提取回收率最低,但不同农药其降低幅度不同,马拉硫磷和乙硫磷降幅最大,分别降至60%和40%。在此基础上,配制了浓度为700mg/L茶多酚和90g/L蔗糖,pH=2.7的混合溶液校准标准曲线。农药浓度在0.1~5μg/L范围内校准曲线线性极好(R2>0.99)。茶饮料中农药加标浓度为1μg/L时,其定量测定值在0.63~1.29μg/L之间。混合基质校准曲线能很好解决纯水标准曲线定量茶饮料有机磷农药HS-SPME-GC法检测中回收率偏低的问题,满足其快速检测的要求。

References

[1]  Wilkes J G,Conte E D,KimY M.Sample preparation for the analysis off lavors and off-flavors in foods[J]. Journal of Chromatography A, 2000, 880: 3-33
[2]  肖 珂, 王 勇, 路 鑫, 孔宏伟, 姚庆红, 许国旺. 固相微萃取-气相色谱/质谱测定工业废水中痕量有机物的研究[J]. 色谱, 2003, 1: 1-3
[3]  王文芳. 顶空固相微萃取气相色谱法测定萝卜中有机氯农药及其代谢物[J]. 公共卫生与预防医学, 2007, 4: 1-3
[4]  孔祥虹. 固相微萃取-气相色谱法测定浓缩苹果汁中的8种有机磷农药残留[J]. 食品科学, 2009, 2: 11-13
[5]  Joana M, Cristina E, Ana L F, Paulo B, Natália R. Analysis of six fungicides and one acaricide in still and fortified wines using solid-phase microextraction-gas chromatography/tandem mass spectrometry[J]. Food Chemistry, 2012, 132: 630-636
[6]  贺利民, 刘祥国, 曾振灵. 气相色谱分析农药残留的基质效应及其解决方法[J]. 色谱, 2008, 26(1): 98-104
[7]  Mercer G E. Determination of 112 halogenated pesticides using gas chromatography/mass spectrometry with selected ion monitoring[J]. Journal of AOAC International, 2005, 88(5): 1452-1461
[8]  Eun-mi An, Han-Seung Shin. Gas Chromatographic Determination of Pesticide Residues Using Electron-capture Detector and Mass Spectrometry[J]. Food Science Biotechnol, 2011, 20(5): 1299-1306
[9]  Rositsa I, Mladenova, Deyana D, Shtereva. Multiresidue determination of pesticides by solid-phase extraction and GC-MS for control of peach production in Bulgaria[J]. International Journal of Environmental Analytical Chemistry, 2011,91(6), 567-575
[10]  Ewa C, Anna S R, Juan M M R, Magdalena S Z. Evaluation of QuEChERS method for the determination of organochlorine pesticide residues in selected groups of fruits[J]. Food Chemistry, 2011, 125: 773-778
[11]  贾秋园, 刘 颖, 李冬如. 市售茶饮料中茶多酚、咖啡因、以及茶氨酸的含量分析[J]. 检测与分析, 2009: 41-44
[12]  Anastassiades M, Matovská K, Lehotay S J. Evaluation of analyte protectants to improve gas chromatographic analysis of pesticides[J]. Journal of Chromatography A, 2003, 1015: 163-184
[13]  Shanker A, Sood C, Kumar V, Ravindranath S D. A modified extraction and clean-up procedure for the detection and determination of parathionmethyl and chlorpyrifos residues in tea[J]. Pest management science, 2001, 5(57): 458-462
[14]  Erney D R, Pawlowski T M, Poole C F. Matrix-induced peak enhancement of pesticides in gas chromatography: Is there a solution? [J]. Hrc-journal of High Resolution Chromatography, 1997, 20(7): 375-378
[15]  陈 莹, 丛佩华, 聂继云, 李 静, 徐国锋. 水果中多菌灵农药残留检测方法的比较与基质效应研究[J]. 分析实验室, 2008, 27: 408-412
[16]  Ulrich S. Solid-phase microextraction in biomedical analysis[J]. Journal of Chromatography A, 2000, 902(1): 167-194
[17]  Basheer C, Alnedhary A A, Rao B S, Lee H K. Determination of organophosphorous pesticides in wastewater samples using binary-solvent liquid-phase microextraction and solid-phase microextraction: A comparative study[J]. Analytica Chimica Acta, 2007, 605(2): 147-152
[18]  袁华丽, 高松亭, 韩朔睽. SPME-GC/MS联用测定室内空气中的挥发性有机物[J]. 南京大学学报-自然科学版, 2001, 6: 1-3
[19]  周 昱, 徐敦明, 陈达捷, 张志刚, 郑向华, 方恩华. 固相微萃取气相色谱法和气相色谱-质谱法测定茶叶中氟虫腈及其代谢物残留[J]. 色谱, 2011, 29(7): 656-661
[20]  Chen Jie, Duan Chunfeng, Guan Yafeng. Sorptive extraction techniques in sample preparation for organophosphorus pesticides in complex matrices[J]. Journal of Chromatography B, 2010, 878: 1216-1225
[21]  Fredericode M R, Paulo R R M, Lidia S O, Fabio S O. Development of a headspace solid-phase microextraction/gas chromatography-mass spectrometry method for determination of organophosphorus pesticide residues in cow milk[J]. Microchemical Journal, 2011, 98: 56-61
[22]  Cristina B, Pablo V R, Matthias O, Ana M, Yolanda P. Analysis of insecticides in honey by liquid chromatography-ion trap-mass spectrometry: Comparison of different extraction procedures[J]. Journal of Chromatography A, 2011, 1218: 4892-4901
[23]  Fang Wu, Wanping Lu, Jinghua Chen, Wei Liua, Lan Zhang. Single-walled carbon nanotubes coated fibers for solid-phase microextraction and gas chromatography-mass spectrometric determination of pesticides in Tea samples[J]. Talanta, 2010, 82: 1038-1043
[24]  冯海强. SPME-GC分析茶饮料和水体中8种有机磷农药[D]. 杭州: 浙江大学, 2010: 16-29
[25]  Hajslova J, Zrostlikova J. Matrix effects in (ultra)trace analysis of pesticide residues in food and biotic matrices[J]. Journal of Chromatography A, 2003. 1000(1-2): 181-197
[26]  Kocourek V, Hajlová J, Holadová K, Poustka J. Stability of pesticides in plant extracts used as calibrants in the gas chromatographic analysis of residues[J]. Journal of Chromarogr A, 1998, 800: 297-304
[27]  Hill A R C, Reynolds S L. Guidelines for in-house validation of analytical methods for pesticide residues in food and animal feeds[J]. Analyst, 1999, 124: 953-958
[28]  Stefan K, Julia S, Guenther K, Speer K. Assessment of sample cleanup and matrix effects in the pesticide residue analysis of foods using postcolumn infusion in liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography A, 2011, 46 (1218): 8399-8410
[29]  段俊彦. 茶叶中有机磷类农药多残留测定时的基质效应研究[D]. 杭州: 中国农业科学院学位论文, 2009: 37-44
[30]  Cédric P, Frédéric H. Evaluation of some parameters affecting troublesome pesticide analysis in gas chromatography-ion-trap mass spectrometry[J]. Journal of Chromatogr A, 2008, 1201: 78-90.
[31]  Barwick V, Ellison S, Lacey S. Evaluation of a solid phase extraction procedure for the determination of pesticide residues in food stuffs[J]. Journal of the Science of Food and Agriculture, 1999, 79(9): 1190-1196
[32]  Arthur C L, Killam L M, Buchholz K D, Pawliszyn J, John R. Automation and optimization of solid-phase microextraction[J]. Analytical chemistry, 1992, 64(17): 1960-1966
[33]  Anastassiades M, Lehotay S J, Stajnbaher D, Schenck F J. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce[J]. Journal of AOAC International, 2003, 86(2): 412-431

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133