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核农学报  2012 

奇球菌属类胡萝卜素提取物的抗氧化活性研究

, PP. 900-905

Keywords: 奇球菌属,类胡萝卜素,抗氧化活性

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Abstract:

从不同种奇球菌属(Deinococci)细菌中分离提取类胡萝卜素,并利用高效液相色谱分析了提取物的组成和性质。通过DPPH自由基和超氧自由基清除试验,比较了不同菌种来源的类胡萝卜素提取物的抗氧化能力,结果发现,D.radiopugnans和D.radiodurans提取物的体外抗氧化能力最强,在浓度为0.6μg/ml时DPPH自由基清除率都达到46%左右;D.radiopugnans提取物的超氧自由基清除活性较强,在浓度为3μg/ml时清除率达到42.99%。同时,采取体外蛋白质氧化抑制试验和脂类氧化抑制能力测定分析比较了不同种类提取物对大分子的保护作用,结果表明D.radiopugnans和D.radiodurans提取物具有较强的生物大分子氧化防护功能。以上研究为从具有极端环境适应能力的奇球菌属筛选抗氧化活性类胡萝卜素,并进一步研究其作用机制提供了基础。

References

[1]  Xu X L, Shen J Y, Dunn C A. The Nudix hydrolases of Deinococcus radiodurans[J]. Molecular Microbiology, 2001, 39(2): 286-290
[2]  Woodall A A, Lee S W, Weesie R J, Jackson M J, Britton G. Oxidation of carotenoids by free radicals: relationship between structure and reactivity[J]. Biochim Biophys Acta, 1997, 1336(1): 33-42
[3]  王业勤, 李勤生. 天然类胡萝卜素研究进展、生产、应用[M]. 北京:中国医药科技出版社, 1996:158
[4]  Tian B, Sun Z, Shen S, Wang H, Jiao J, Wang L, Hu Y, Hua Y. Effects of carotenoids from Deinococcus radiodurans on protein oxidation[J]. Letters in Applied Microbiology, 2009, 49:689-694
[5]  Tian B, Sun Z, Xu Z, Hua Y. Chemiluminescence analysis of the prooxidant and antioxidant effects of epigallocatechin-3-gallate[J]. Asia Pacific Journal of Clinical Nutrition, 2007, 16 (Suppl) 1:153-157
[6]  Dominguez-Rebolledo A E, Martinez-Pastor F, Fernandez-Santos M R, del Olmo E, Bisbal A, Ros-Santaella J L, Garde J J. Comparison of the TBARS assay and BODIPY C11 probes for assessing lipid peroxidation in red deer spermatozoa[J]. Reproduction in Domestic Animals-Zuchthygiene, 2010, 45:e360-368
[7]  Lemee L,Peuchant E,Clerc M,Brunner M, Pfander H. Deinoxanthin: a new carotenoid isolated from Deinococcus radiodurans[J]. Tetrahedron, 1997: 919-926
[8]  Jung S, Joe M, Im S, Kim D, Lim S. Comparison of the genomes of deinococcal species using oligonucleotide microarrays[J]. Journal of Microbiology and Biotechnology, 2010, 20:1637-1646
[9]  Seo H J, Lee Y N. Occurrence of thioredoxin reductase in Deinococcus species, the UV resistant bacteria[J]. Journal of Microbiology, 2006, 44:461-465
[10]  Masters C I, Murray R G, Moseley B E, Minton K W. DNA polymorphisms in new isolates of 'Deinococcus radiopugnans’[J]. Journal of General Microbiology, 1991, 137:1459-1469
[11]  Filipkowski P, Koziatek M, Kur J. A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans[J]. Extremophiles: Life under Extreme Conditions, 2006, 10:607-614
[12]  Sun Z, Shen S, Tian B, Wang H, Xu Z, Wang L, Hua Y. Functional analysis of gamma-carotene ketolase involved in the carotenoid biosynthesis of Deinococcus radiodurans[J]. FEMS Microbiology Letters, 2009, 301:21-27
[13]  Tian B, Xu Z, Sun Z Lin J, Hua Y. Evaluation of the antioxidant effects of carotenoids from Deinococcus radiodurans through targeted mutagenesis, chemiluminescence, and DNA damage analyses [J].Biochim Biophys Acta, 2007, 1770(6): 902-911
[14]  Tian B, Wang H, Ma X, Hu Y, Sun Z, Shen S, Wang F, Hua Y. Proteomic analysis of membrane proteins from a radioresistant and moderate thermophilic bacterium Deinococcus geothermalis[J]. Molecular Biosystems, 2010, 6:2068-2077

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