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核农学报  2013 

花生种子芽期耐低温相关基因克隆

, PP. 152-157

Keywords: 花生,耐低温,相关基因,差异表达

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Abstract:

利用GeneFishing技术获得耐低温花生品种花育44号种子在常温与低温诱导下的差异表达基因(differentiallyexpressedgenes,DEGs)ACP20和ACP2。BLAST比对后发现ACP20基因与花生油质蛋白基因同源性达99%,ACP2基因与花生nifU-like(铁硫簇蛋白)蛋白mRNA同源性达99%,说明油质蛋白和nifU-like蛋白可能与花生对低温逆境胁迫的抗性有关。通过RACE技术从该品系获得相关基因的完整编码区,对其进行生物学功能预测,为探讨花生耐低温分子机制乃至培育耐低温花生新品种奠定了基础。

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