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核农学报  2013 

七种培养基对黄曲霉分离效果的比较

, PP. 208-212

Keywords: 黄曲霉菌,土壤,分离

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Abstract:

为了筛选出适宜黄曲霉分离的培养基,比较了7种常用于霉菌分离的培养基对10个花生土壤样品的黄曲霉分离效果,这7种培养基为氯硝胺18%甘油培养基(DG18)、高盐察氏培养基(SCDA)、马铃薯琼脂培养基(PDA)、改良的孟加拉红培养基(M-RB)、YES培养基、孟加拉红培养基(RB)、氯硝胺孟加拉红琼脂(DRBC)。研究发现7种培养基对黄曲霉菌培养效果差别很大,培养效果最好的是DG18,黄曲霉的培养数是PDA的7.3倍。同时DG18能很好的抑制毛霉的生长,与DRBC培养基相比,能抑制81%的毛霉菌生长。因此DG18培养基能最大限度的保证黄曲霉的生长分离,同时也能更好地抑制毛霉的生长,更适用于黄曲霉种群、遗传方面的研究应用。

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