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核农学报  2013 

两个低植酸大豆突变体中肌醇-3-磷酸合成酶MIPS1基因表达特性的研究

, PP. 286-292

Keywords: 大豆,低植酸,MIPS1基因,表达特性

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Abstract:

肌醇-3-磷酸合成酶(MIPS)基因是植酸合成代谢途径中重要的功能基因。本研究以两个大豆低植酸突变体Gm-lpa-TW-1、Gm-lpa-ZC-2以及其野生型亲本台湾75和浙春3号为材料,分析了MIPS1基因在其根、茎、叶、花和发育种子中转录水平上的表达特性。结果表明在大豆发育的种子中MIPS1基因的表达表现为由弱到强再逐渐减弱的表达特性,在开花后22d的种子内,基因的表达达到高峰,此后逐渐减弱。突变体Gm-lpa-TW-1和野生型亲本台湾75的根、茎、叶和花中MIPS1基因的表达均较弱,但突变体Gm-lpa-TW-1的表达量略高于野生型亲本。与野生型亲本相比较,在开花后的22d,突变体Gm-lpa-TW-1发育种子中MIPS1基因的表达峰极显著的降低,仅为野生型的25%左右,而且在整个种子发育期间MIPS1基因的表达均较弱。突变体Gm-lpa-ZC-2和浙春3号的根、茎、叶和花中MIPS1基因的表达较弱,突变型和野生型之间没有显著的差异,但在种子发育的各个时期突变体Gm-lpa-ZC-2的表达量显著的高于野生型亲本浙春3号。在两个低植酸突变体发育的种子中MIPS1基因的表达与野生型亲本相比均发生了显著的变化,表明基因突变对MIPS1基因的表达均造成了显著的影响,在Gm-lpa-TW-1中表现为MIPS1基因表达的下调,而Gm-lpa-ZC-2中则表现为上调。

References

[1]  Walker D R, Scaboo A M, Wilcox J R. Genetic mapping of loci associated with seed phytic acid content in CX1834-1-2 soybean [J].Crop Science, 2006, 46:390-397
[2]  Xu X H, Zhao H J, Liu Q L, Frank T, Engel K H, An G, Shu Q Y. Mutations of the multi-drug resistance-associated protein ABC transporter gene 5 result in reduction of phytic acid in rice seeds [J]. Theoretical and Applied Genetics, 2009, 119:75-83
[3]  Yuan F J, Zhu D H, Tan Y Y, Dong D K, Fu X J, Zhu S L, Li B Q, Shu Q Y. Identification and characterization of the soybean IPK1 ortholog of a low phytic acid mutant reveals an exon-excluding splice-site mutation [J] Theoretical and Applied Genetics,2012,125:1413-1423
[4]  Majumder A L, Biswas B B. Metabolism of inositol phosphates: 5. Biosynthesis of inositol phosohates during ripening of mung bean seeds [J]. India Journal of Express Biology. 1973, 11:120-123
[5]  Coelho C M M, Benedito V A, Figueira A, Vitorello V A, Azevedo R A. Variation in the enzyme activity and gene expression of myo-inositol-3-phosphate synthase and phytate accumulation during seed development in common bean [J]. Acta Physiology Plant, 2007, 29:265-271
[6]  Suzuki M, Tanaka K, Kuwano M, Yoshida K T. Expression pattern of inositol phosphate-related enzymes in rice (Oryza sativa L): Implications for the phytic acid biosynthetic pathway[J]. Gene, 2007, 405:55-64
[7]  Veum T L, Ledoux D R, Robay V, Etrl D S. Low-phytic corn improves nutrient utilization of growing pig [J]. Journal of Animal Science, 2001, 79:2873-2880
[8]  Li Y C, Ledoux D R, Veum T L, Raboy V, Ertl D S. Effect of low phytic acid corn on phosphorus utilization, performance, and bonemineralization in broiler chicks [J]. Poultry Science, 2000, 79:1445-1450
[9]  Yuan F J, Zhao H J, Ren X L, Zhu S L, Fu X J, Shu Q Y. Generation and characterization of two novel low phytate mutations in soybean (Glycine max L. Merr)[J]. Theoretical and Applied Genetics, 2007,115:945-957
[10]  Liu Q L, Xu X H, Ren X L, Fu H W, Shu Q Y. Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.)[J]. Theoretical and Applied Genetics, 2007, 114:803-814
[11]  Shi J R, Wang H Y, Hazebroek J, Ertl D S, Harp T. The maize low-phytic acid 3 encodes a myo-insitol kinase that plays a role in phytic acid biosynthesis in developing seeds [J]. Plant Journal, 2005, 42:708-719
[12]  Hize W D, Carlson T J, Kerr P S, Sebastian S A. Biochemical and molecular characterization of a mutation that confers a decreased raffinosaccharide and phytic acid phenotype on soybean seeds [J]. Plant Physiology, 2002, 128:650-660
[13]  Kim S I, Andaya J W, Newman J W, Goyal S S, Tai T H. Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK [J]. Theoretical and Applied Genetics, 2008, 117:1291-1301
[14]  Shi J R, Wang H Y, Wu Y S, Hazebroek J, Meeley R B, Ertl D S. The maize low-phytic acid mutant lpa2 is caused by mutation in an inositol phosphate kinase gene [J]. Plant Physiology, 2003, 131:507-515
[15]  Nunes A C S, Vianna G R, Cuneo F, Amaya F J, Capdeville G, Rech E L, Arogao F J L. RANi-mediated silencing of the myo-inositol phosphate synthase gene (GmMIPS) in trans-genic soybean inhibited seed development and reduced phytate content [J]. Planta, 2006,224(1):125-132
[16]  Chappell A S, Scaboo A M, Wu X, Pantalone V R, Bilyeu K D.Characterization of the MIPS gene family in Glycine max [J]. Plant Breeding, 2006, 125:493-500
[17]  Shi J, Ertl D S, Wang H Y, Li B L, Faller M, Schellin K. Maize multidrug resistance-associated protein polynucleotides and methods of use [P]. US Patent, 20060143728
[18]  Shunkla S, Vantoai T T, Pratt R C. Expression and nucleotide sequence of an INS(3)P1 synthase gene associated with low-phytate kernels in maize (Zea mays L.) [J]. Journal of Agricultural and Food Chemistry, 2004, 52:4565-4570

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