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核农学报  2013 

基于双特异性单克隆抗体的克百威和三唑磷多残留ELISA分析方法研究及在环境样品中应用

Keywords: 双特异性单克隆抗体,克百威,三唑磷,多残留检测,ELISA

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Abstract:

采用杂交-杂交瘤技术制备抗克百威和三唑磷的双特异性单克隆抗体并进行纯化,通过对反应模式、工作浓度、包被缓冲液、有机溶剂、离子强度、pH值等条件优选,建立优化的ELISA分析方法,对克百威和三唑磷的线性回归方程分别为Y=20.009ln(x)-9.9293(R2=0.9959)和Y=19.486ln(x)+39.752(R2=0.9945),抑制中浓度I50分别为20ng·mL-1和1.69ng·mL-1,最低检测限I20分别为4.46ng·mL-1和0.36ng·mL-1。建立的ELISA分析方法对水、土壤等环境样品中克百威和三唑磷的平均添加回收率介于88.4%~117%之间,说明所制备的双特异性单克隆抗体和建立的ELISA分析方法可靠,可应用于克百威和三唑磷的多残留免疫分析。

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