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核农学报  2013 

吡虫啉对褐飞虱DNA多态性的影响

DOI: 10.11869/hnxb.2013.05.0584, PP. 584-590

Keywords: 吡虫啉,褐飞虱,ISSR,AFLP,DNA多态性,抗药性

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Abstract:

吡虫啉在对抗水稻褐飞虱虫害中大量使用。本研究结合ISSR和AFLP分子标记技术检测吡虫啉对褐飞虱的遗传多样性影响。利用20个ISSR引物对连续施用吡虫啉的褐飞虱9个世代的19份材料扩增,共获得49个位点,其中只有1个位点出现特异性条带。利用25对AFLP引物对供试材料扩增,结果共有111个扩增位点,呈现多态性条带的位点11个。共获得59个多态性条带。其中多态性条带比例最高的是G8(10.2%),比例最低的是G1(1.69%)。多态性条带比例变化趋势表明,在筛选的早期G1、G2和G3世代,褐飞虱对吡虫啉的应答较快;在筛选的中期G4、G5和G6世代,褐飞虱对吡虫啉的应答呈现较大幅度的波动,变化范围在1.69%~8.47%之间;在筛选的晚期G7,G8和G9世代,褐飞虱对吡虫啉的应答波动幅度较小,趋于一个相对稳定的水平,变化范围在6.78%~10.2%之间。本研究结果表明,吡虫啉的连续使用,可能会造成褐飞虱DNA的突变,从而使害虫产生抗药性。本研究结果为揭示褐飞虱抗药性产生的分子机理提供了重要信息。

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