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核农学报  2013 

甘薯种质资源的SRAP鉴定及遗传多样性分析

DOI: 10.11869/hnxb.2013.05.0568, PP. 568-575

Keywords: 甘薯,遗传多样性,聚类分析,SRAP,农艺性状

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Abstract:

为了拓宽甘薯育成品种的遗传背景,筛选优良亲本,提高育种效率,本研究利用SRAP标记对48份主要甘薯种质资源进行了遗传多样性分析。结果表明,随机选用的37对SRAP引物中29对引物具有多态性,引物多态性比率为78.4%,共获得126条多态性谱带,平均每对引物产生4.3条多态性谱带,表现出较高的多态性。48份种质材料的SRAP遗传距离为0.037~0.601,当遗传距离L1=0.46时,48份材料被聚为6个类群,包括1个复合大类群和5个独立类群,其中第Ⅰ复合大类群又包括7个亚类群,聚类结果与系谱吻合性较好。采用5个重要农艺性状对供试材料进行了聚类分析,L1=3.20时,48份材料也可被聚为6个类群,聚类结果与依据SRAP标记聚类分析的结果差异较大。甘薯种质资源间的遗传差异与地理来源无必然联系。

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