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核农学报  2013 

环境胁迫诱导的花生全长cDNA文库的构建及序列分析

DOI: 10.11869/hnxb.2013.05.0545, PP. 545-551

Keywords: 花生,胁迫,SMART技术,全长cDNA文库,抗逆基因

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Abstract:

以花生新品种闽花6号为材料,用生物和非生物因素胁迫处理植株,采用SMART技术构建了花生受低温、干旱、各种激素、缺钙及黄曲霉等胁迫诱导的混合全长cDNA文库,原始文库滴度为8.86×106cfu,重组率达97.6%,插入片段多在1~2kb之间,平均大小为1300bp。该cDNA文库的构建可用于花生抗逆基因的发掘,为揭示花生抗逆分子机理及花生品种改良奠定基础。

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