Leung D W, Chen E, Goeddel D V. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction[J]. Technique, 1989, 1: 11-15
[2]
Cadwell C, Joyce G F. Randomization of genes by PCR mutagenesis[J]. PCR Methods and Applications. 1992, 2: 28-33
[3]
沈 同, 王镜岩. 生物化学(第二版上册)[M].北京:高等教育出版社,2002,337-338
[4]
Sinha N K, Haimes M D. Molecular mechanisms of substitution mutagenesis: an experimental test of the Watson-Crick and Topal-Fresco models of base mispairings. The journal of biological chemistry[J], 1981, 256(20): 10671-10683
[5]
Lehtovaara P M, Koivula A K, Bamford J, Knowles J K C. A new method for random mutagenesis for complete genes: enzymatic generation of mutant libraries in vitro[J].Protein Engineering, 1988, 2(1): 63-68
[6]
Watson J D, Crick F H C. Genetical implications of the structure of deoxyribonucleic acid[J]. Nature, 1953, 171: 964-967
[7]
Keohavong P, Thilly W G. Fidelity of DNA polymerases in DNA amplification[C]. Proceedings of the National Academy of Sciences of the United States of America, 1989, 86: 9253-9257
[8]
Bernstein C, Bernstein H, Mufti S, Strom B. Stimulation of mutation in phage T 4 by lesions in gene 32 and by thymidine imbalance[J]. Mutation Research, 1972, 16 (2): 113-119
[9]
Saiki R K, Scharf S, Faloona F, Mullis K B, Horn G T, Erlich H A, Arnheim N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia[J]. Science, 1985, 230: 1350-1354
[10]
Eckert K A, Kunkel T A. DNA polymerase fidelity and the polymerase chain reaction[J]. PCR Methods and Applications, 1991, 1: 17-24
[11]
Chaput J C, Woodbury NW, Stearns L A. Williams BA. Creating protein biocatalysts as tools for future industrial applications[J]. Expert Opinion on Biological Therapy, 2008, 8: 1087-1098
[12]
Hibner U, Alberts B. Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophagemulti-enzyme complex[J]. Nature, 1980, 285: 300-305
[13]
Ling L L, Keohavong P, Dias C, Thilly W G. Optimization of the polymerase chain reaction with regard to fidelity: Modified T7, Taq, and Vent polymerases[J]. PCR Methods and Applications, 1991, 1: 63-69
[14]
Cadwell R C, Joyce G F. Mutagenic PCR[J]. PCR Methods and Applications, 1994, 3 (6): 136-140
[15]
Riedel K H, Wingfied B D, Britz T J. Combined influence of magnesium concentration and polymerase chain reaction specificity enhancers[J]. FEMS Microbiol Letters, 1992, 92: 69-72
[16]
Zhao H, Giver L, Shao Z, Affholter J A, Arnold F H. Molecular evolution by staggered extension process (StEP) in vitro recombination[J]. Nature Biotechnology, 1998, 16(3): 258-261
[17]
Miyazaki K, Takenouchi M, Kondo H, Noro N, Suzuki M, Tsuda S. Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution[J]. Journal of Biological Chemistry, 2006, 281 (15): 10236-10242
[18]
An Y F, Ji J, Wu W, Huang R, Wei Y, Xiu Z. Random mutagenesis and recombination of sam1 gene by integrating error-prone PCR with staggered extension process[J]. Biotechnology Letters. 2008, 30(7): 1227-1232
[19]
Claveau S, Sasseville M, Beauregard M. Alcohol-Mediated Error-Prone PCR[J]. DNA and Cell Biology. 2004, 23(11): 789-795
[20]
Biles B D, Connolly B A. Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR[J]. Nucleic Acids Research, 2004, 32: e176
[21]
Tindall K T, Kunkel T A. Fidelity of DNA synthesis by the thermus aquaticus DNA polymerase[J]. Biochemistry, 1988, 27, 6008-6013
[22]
Saiki R K, Gelfand D H, Stoffel S, Scharf S J, Higuchi R, Horn G T, Mullis K B, Erlich H A. Primer-directed enzymatic amplificiation of DNA with a thermostable DNA polymerase[J]. Science, 1988, 239: 487-491
[23]
Cline J, Braman J C, Hogrefe H H. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerase[J].Nucleic Acids Research, 1996, 24: 3546-3551
[24]
Beckman R A, Mildvan A S, Loeb L A. On the fidelity of DNA replication: manganese mutagenesis in vitro[J]. Biochemistry, 1985, 24: 5810-5817
[25]
Hall Z W, Lehman I R. An in vitro transversion by a mutationally altered T4-induced DNA polymerase[J]. Journal Molecular Biology, 1968, 36: 321-333
[26]
Kunkel T A, Loeb L A. On the fidelity of DNA replication, Effect of divalent metal ion activators and deoxyribonucleoside triphosphate pools on in vitro mutagenesis[J]. The Journal of Biological Chemistry., 1979, 254: 5718-5725
[27]
Fersht A R. Fidelity of replication of phage φ×174 DNA by DNA polymerase III holoenzyme: Spontaneous mutation by misincorporation[C]. Proceedings of the National Academy of Sciences of the United States of America, 1979, 76 (10): 4946-4950
Stemmer W P C. Rapid evolution of a protein in vitro by DNA shuffling[J]. Nature, 1994, 370 (6488): 389-391
[30]
Zhao H, Arnold F H. Directed evolution converts subtilisin E into a functional equivalent of thermitase[J]. Protein Engineering, 1999, 12(1): 47-53
[31]
Potocki-Veronese G, Putaux J L, Dupeyre D, Albenne C, Remaud-Simeon M, Monsan P, Buleon A. Amylose synthesized in vitro by amylosucrase: morphology, structure, and properties[J]. Biomacromolecules, 2005, 6: 1000-1011
[32]
Zhou M, Xu H L, Wei X L, Ye Z Q, Wei L P, Gong W M, Wang Y Q, Zhu Z. Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate3-phosphate synthase using a directed evolution strategy[J], Plant Physiology, 2006, 140(1): 184-195
Arnold F H, Wintrode P L, Miyataki K, Gershenson A. How enzymes adapt: lessons from directed evolution[J]. Trends in Biochemical Sciences, 2001, 26: 100-106
