全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2013 

转rd29A-ICE1冷诱导基因水稻提高抗寒性研究

DOI: 10.11869/hnxb.2013.06.0731, PP. 731-735

Keywords: 水稻,ICE1基因,rd29A启动子,遗传转化,耐寒性

Full-Text   Cite this paper   Add to My Lib

Abstract:

本研究以pCAMBIA1300质粒为基础,构建了植物冷诱导表达载体pCAMBIA1300-rd29A-ICE1,利用农杆菌介导法导入粳稻品种空育131中。PCR、RT-PCR和Southern杂交检测结果表明,ICE1基因已经成功整合到水稻基因组中,并正常表达。与对照相比,低温处理后超表达ICE1基因的水稻转基因株系存活率和脯氨酸含量明显增加,丙二醛含量积累速率明显下降,提高了抗低温胁迫能力。

References

[1]  Thomashow M F. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1999, 50: 571-599
[2]  Chinnusamy V, Ohta M, Kanrar S, Lee B H, Hong X, Agarwal M, Zhu J K.ICE1: a regulator of cold-induced transcriptome and freezing tolerance in Arabidopsis[J]. Genes&Development, 2003, 17: 1043-1054
[3]  林元震,张志毅,刘纯鑫,郭海,朱保庆,陈晓阳. 甜杨抗冻转录因子ICEl基因的in silico克隆及其分析[J]. 分子植物育种,2007, 5(3): 424-430
[4]  黄家权. 抗寒基因的克隆及根癌农杆菌介导的ICE1基因转化柑橘的研究[D].武汉:华中农业大学,2005
[5]  张玉,蒋欣梅,于锡宏.农杆菌介导的ICE1基因转化番茄的研究[J]. 作物杂志,2010,5: 51-55
[6]  郑银英,崔百明,常明进,彭明.转拟南芥ICEl基因增强烟草抗寒性的研究[J]. 西北植物学报,2009,29(1): 75-79
[7]  Hiei Y, Ohta S, Komar T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA[J]. The Plant Journal, 1994, 6(2): 271-282
[8]  南丽丽,师尚礼,朱新强,郭全恩. 田间越冬期不同根型苜蓿根系的生理生化特性[J].核农学报,2011,25(2): 369-374
[9]  冯绪猛,罗时石,胡建伟,吴进才,王泽港,马飞. 农药对水稻叶片丙二醛及叶绿素含量的影响[J].核农学报,2003,17(6): 481-484
[10]  Guy C L, Niemi K J, Brambl R. Altered gene expression during cold acclimation of spinach[J]. Proceedings of the National Academy of Sciences, 1985, 82: 3673-3677
[11]  Ito Y, Katsura K, Maruyama K, Taji T, Kobayashi M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K.Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice[J]. Plant and Cell Physiology, 2006, 41: 41-153
[12]  张宁,司怀军,王蒂.拟南芥rd29A基因启动子克隆及其在马铃薯抗胁迫转基因中的应用[J].作物学报, 2005, 31(2):159-164
[13]  Pellegrineschi A, Reynolds M, Pacheco M, Brito RM, Almeraya R, Yamaguchi-Shinozaki K, Hoisington D.Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions[J]. Genome, 2004, 47: 493-500
[14]  轩春雷,肖向文,李晓波,祝建波.盐芥ICE1转录因子的克隆及生物信息学分析[J]. 生物技术通报,2010,11: 108-114
[15]  Badawi M, Reddy Y V, Agharbaoui Z, Tominaga Y, Danyluk J, Sarhan F, Houde M. Structure and functional analysis of wheat ice (Inducer of CBF Expression) genes[J]. Plant Cell Physiology, 2008, 49(8): 1237-1249
[16]  Lee B H, Henderson D A, Zhu J K.The arabidopsis cold-responsive transcriptome and its regulation by ICE1[J]. The Plant Cell, 2005, 17: 3155-3175

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133