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核农学报  2013 

水稻蜡质合成相关基因OsGL1-5的表达模式及逆境响应特征分析

DOI: 10.11869/hnxb.2013.07.0938, PP. 938-945

Keywords: 水稻,OsGL1-5,表达,逆境

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Abstract:

通过生物信息学方法对水稻蜡质合成相关基因OsGL1-5启动子序列特点进行分析;并以水稻中花11不同组织和发育时期的穗子为材料,通过半定量RT-PCR及mRNA原位杂交技术分析OsGL1-5的时空表达特征;同时,分别以200mmol·L-1NaCl、100μmol·L-1ABA和1.0%H2O2处理水稻幼苗,通过半定量RT-PCR分析逆境胁迫下OsGL1-5的表达模式。生物信息学分析表明,OsGL1-5启动子中包括大量与干旱、冷、盐等多种逆境胁迫相关的调控序列。时空表达特征分析表明,OsGL1-5主要在不同发育时期的穗子中表达,且表达部位集中在稃片的毛状体和花药绒毡层中,叶中表达量相对较低,而茎和根中没有检测到表达。NaCl、ABA以及H2O2等逆境胁迫下,OsGL1-5表达量在不同处理时间有所增加。

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