全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2013 

甘蓝类植物小孢子培养及植株再生研究

DOI: 10.11869/hnxb.2013.07.0929, PP. 929-937

Keywords: 甘蓝类植物,小孢子培养,胚胎发生,植株再生,倍性鉴定

Full-Text   Cite this paper   Add to My Lib

Abstract:

对3个甘蓝类植物(甘蓝、青花菜、羽衣甘蓝)19个品种小孢子胚胎发生和植株再生的影响因素进行了研究,结果表明,基因型不仅影响供试材料的小孢子胚状体发生频率,也影响胚状体的质量;4℃低温预处理0~2d结合32.5℃热激1d,能显著提高供试基因型小孢子出胚率。胚状体先在NLN-13液体培养基中培养25d,有利于胚状体分化成苗;后在B5固体分化培养基中添加1%~1.2%琼脂,可有效促进胚状体萌发和植株再生。小孢子再生植株先使用流式细胞仪在生长初期鉴定倍性,后对单倍体植株采用200mg·L-1秋水仙碱浸根处理20h,并用流式细胞仪鉴定,此方法可快速、有效地获得双单倍体植株。

References

[1]  陈秋芳,贾宏汝.十字花科植物的研究价值及开发利用前景[J].安徽农业科学,2007,35(34):183-185
[2]  王国槐,官春云,陈社员,刘本坤,刘忠松.十字花科芸薹属种间杂种营养优势的利用研究[J].作物学报,2003,29(1):54-58
[3]  袁素霞,刘玉梅,方智远,杨丽梅,庄木,张扬勇,孙培田.结球甘蓝和青花菜小孢子胚植株再生[J].植物学报,2010,45(2):226-232
[4]  严准,田志宏,孟金陵.甘蓝游离小孢子培养的初步研究[J].华中农业大学学报,1999,18(1):5-7
[5]  顾宏辉,张冬青,周伟军.换培养液和秋水仙碱处理对白菜型油菜小孢子胚胎发生的影响[J].作物学报,2004,30(1):78-81
[6]  何杭军,王晓武,王炳良.芥蓝游离小孢子培养初报[J].园艺学报,2004,31(2):239-240
[7]  姜凤英, 冯辉.羽衣甘蓝游离小孢子培养初报[J].园艺学报,2005,32(5):884
[8]  Zhou W J, Hagberg P, Tang G X. Increasing embryogenesis and doubling efficiency by immediate colchicines treatment of isolated microspores in spring Brassica napus [J]. Euphytica, 2002, 128: 27-34
[9]  张振超,张蜀宁,张伟,张红亮.四倍体不结球白菜的诱导及染色体倍性鉴定[J].西北植物学报,2007,27(1):28-32
[10]  张延国,王晓武.小孢子培养技术在青花菜上的应用[J].中国蔬菜, 2005,1(6):7-8
[11]  陈文辉,方淑桂,曾小玲,朱朝辉,廖晓珍.甘蓝和青花菜杂种小孢子培养[J].热带亚热带植物学报,2006,14(4):321-326
[12]  赵前程,吉立柱,蔡荣旗,刘莉莉,佟志强,孙德岭.花椰菜游离小孢子培养及植株再生研究[J].华北农学报,2007,22(6):65-68
[13]  周志国,龚义勤,王晓武,柳李旺,张延国.不同萝卜品种游离小孢子的诱导及培养体系优化研究[J].西北植物学报,2007,27(1):33-38
[14]  Touraev A, Indrianto A, Wratschko I, Vicente O, Heberle-Bors E. Efficient microspore embryogenesis in wheat (Triticum aestivum L.), induced by starvation at high temperature [J]. Sex Plant Reprod, 1996, 9: 209-215
[15]  栗根义,高睦枪,赵秀山.大白菜游离小孢子培养[J].园艺学报,1993,20(2):167-170
[16]  刘公社,李岩,刘凡.高温对大白菜小孢子培养的影响[J].植物学报,1995,37(2):140-146
[17]  Custers J B M, Cordewener J H G, Nollen Y, Dons H J M, Campagne M M V. Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus [J]. Plant Cell Reports, 1994, 13: 267-271
[18]  Gu H H, Hagberg P, Zhou W J. Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.) [J]. Plant Growth Regulation, 2004, 42: 137-143
[19]  Sopory S K, Munchi M. Anther culture. In: Monhanjain SM, Sopory SK, Veilleux RE (eds). In vitro haploid production in higher plants [M]. Dordrecht: Kluwer Academic Publishers, 1996:145-176
[20]  Sato T, Nishio T, Hirai M. Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris ssp.Pekinensis) [J]. Plant Cell Reports, 1989, 8: 486-488
[21]  Pechan P M, Bartels D, Brown D C W, Schell J. Messenger-RNA and protein changes associated with induction of Bassica microspore embryogenesis [J]. Planta, 1991, 184: 161-165
[22]  余凤群,刘后利.供体材料和培养基成分对甘蓝型油菜小孢子胚状体产量的影响[J].华中农业大学,1995, 14(4):327-331
[23]  Kott L S, Polsoni L, Ellis B, Beversdorf W D. Autotoxicity in isolated microspores of Brassica napus [J]. Canadian Journal of Botany, 1988, 66: 1665-1670
[24]  Zaki M. Modification of cell development in vitro: The efficient of colchicines on anther and isolated microspore culture in Brassica napus [J]. Plant Cell Tissue and Organ Culture, 1995, 40: 255-270
[25]  Lichter R. Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species [J]. Plant Breeding, 1989, 103: 119-123
[26]  Chen Q, Armstrong K. Genomic in situ hybridization in Avena sativa [J]. Genome, 1994, 37: 607-612
[27]  周伟军, 唐桂香, 张国庆, Hagberg P.甘蓝型油菜小孢子秋水仙素处理提高双单倍体频率研究[J]. 中国农业科学,2002,35(4):410-414
[28]  Roux N, Toloza A, Radecki Z, Zapata-Arias FJ, Dole?el J. Rapid detection of aneuploidy in Musa using flow cytometry [J]. Plant Cell Reports, 2003, 21: 483-490
[29]  Greilhuber J, Temsch E M, Loureiro J C M. Nuclear DNA content measurement in flow cytometry with plant cells [M]. Weinheim:Wiley VCH, 2007: 67-101
[30]  Dias J S. Effect of incubation temperature regimes and culture medium on broccoli microspore culture embrogenesis [J]. Euphytica, 2001, 119: 389-394

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133