全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2013 

核盘菌和灰葡萄孢菌CCCH锌指蛋白基因的鉴定和比较分析

DOI: 10.11869/hnxb.2013.08.1118, PP. 1118-1124

Keywords: CCCH锌指蛋白,核盘菌,灰葡萄孢菌,基因组

Full-Text   Cite this paper   Add to My Lib

Abstract:

CCCH锌指蛋白(zincfingerproteins)广泛分布于动物、植物和微生物之中,在RNA代谢过程中起着重要作用,通过鉴定核盘菌和灰葡萄孢菌Zf基因,为基因克隆、表达分析和功能研究奠定基础。本研究以核盘菌和灰葡萄孢菌全基因组序列为材料,运用生物信息学的手段开展Zf基因的全基因组鉴定和分析。经分析,核盘菌和灰葡萄孢菌各有11个Zf基因,它们的开放性阅读框全长分别为633~3741bp和633~3486bp;共检测到55个CCCH锌指基序,以C-X7-C-X5-C-X3-H(22个)和C-X8-C-X5-C-X3-H(21个)最为常见,其中C-X8-C-X6-C-X3-H和C-X7-C-X6-C-X3-H为2种新发现的基序类型;进化分析结果表明,22个Zf基因聚为4组,除SS1G_13631、SS1G_09360、BC1T_00310和BC1T_05217外,核盘菌和灰葡萄孢菌的Zf基因分别位于不同的小分支,该结果与Zf基因的基因结构、基序数量和分布情况一致。

References

[1]  Boland G J, Hall R. Index of plant hosts of Sclerotinia sclerotiorum[J]. Canadian Journal of Plant Pathology, 1994, 16: 93-108
[2]  Bolton M D, Thomma B P H J, Nelson B D. Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen[J]. Molecular Plant Pathology, 2006, 7: 1-16
[3]  Garg H, Li H, Sivasithamparam K, Kuo J, Barbetti M J. The infection processes of Sclerotinia sclerotiorum in cotyledon tissue of a resistant and a susceptible genotype of Brassica napus[J]. Annals of Botany, 2010, 106(6): 897-908
[4]  Peluffo L, Lia V, Troglia C, Maringolo C, Norma P, Escande A, Esteban Hopp H, Lytovchenko A, Fernie A R, Heinz R, Carrari F. Metabolic profiles of sunflower genotypes with contrasting response to Sclerotinia sclerotiorum infection[J]. Phytochemistry, 2010, 71(1): 70-80
[5]  钟军, 李栒, 官春云. 芸薹属植物抗菌核病的研究进展[J]. 中国油料作物学报, 2002, 24(3): 78-81
[6]  Hoffman D D, Hartman G L, Mueller D S, Leitz R A, Nickell C D, Pedersen W L. Yield and seed quality of soybean cultivars infected with Sclerotinia sclerotiorum[J]. Plant Disease, 1998, 82: 826-829
[7]  Tonneijck A E G, Leone G. Changes in susceptibility of bean leaves (Phaseolus vulgaris) to Sclerotinia sclerotiorum and Botrytis cinerea by pre-inoculative ozone exposures[J]. European Journal of Plant Pathology, 1993, 99(5-6): 313-322
[8]  Singh B K, Singh G P, Singh R B, Dabbas M R. Management of stem rot of broccoli caused by Sclerotinia sclerotiorum through cultural practiced[J]. International Journal of Plant Pathology, 2011, 4(1): 20-22
[9]  Williamson B, Tudzynski B, Tudzynski P, van Kan J A. Botrytis cinerea: the cause of grey mould disease[J]. Molecular Plant Pathology, 2007, 8(5): 561-580
[10]  李伟, 陈怀谷, 张爱香, 陈丽华, 姜伟丽. 核盘菌和灰葡萄孢基因组中的简单重复序列分析[J]. 遗传, 2007, 29(9): 1154-1160
[11]  Amselem J, Cuomo C A, van Kan J A, Viaud M, Benito E P, Couloux A, Coutinho P M, de Vries R P, Dyer PS, Fillinger S, Fournier E, Gout L, Hahn M, Kohn L, Lapalu N, Plummer K M, Pradier J M, Quévillon E, Sharon A, Simon A, ten Have A, Tudzynski B, Tudzynski P, Wincker P, Andrew M, Anthouard V, Beever R E, Beffa R, Benoit I, Bouzid O, Brault B, Chen Z, Choquer M, Collémare J, Cotton P, Danchin E G, Da Silva C, Gautier A, Giraud C, Giraud T, Gonzalez C, Grossetete S, Güldener U, Henrissat B, Howlett B J, Kodira C, Kretschmer M, Lappartient A, Leroch M, Levis C, Mauceli E, Neuvéglise C, Oeser B, Pearson M, Poulain J, Poussereau N, Quesneville H, Rascle C, Schumacher J, Ségurens B, Sexton A, Silva E, Sirven C, Soanes D M, Talbot N J, Templeton M, Yandava C, Yarden O, Zeng Q, Rollins J A, Lebrun M H, Dickman M. Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea[J]. PLoS Genetics, 2011, 7(8): e1002230
[12]  Ciftci-Yilmaz S, Mittler R. The zinc finger network of plants[J]. Cellular and Molecular Life Sciences, 2008, 65(7-8): 1150-1160
[13]  李晓波, 张俊武. 真核生物中锌指蛋白的结构与功能[J]. 中国生物化学与分子生物学报, 2009, 25(3): 206-211
[14]  Berg J M, Shi Y. The galvanization of biology: a growing appreciation for the roles of zinc[J]. Science, 1996, 271(5252): 1081-1085
[15]  秦智慧, 杨青川, 晁跃辉, 康俊梅. CCCH型锌指蛋白研究进展[J]. 生物技术通报, 2010, 8: 1-6
[16]  Hall T M. Multiple modes of RNA recognition by zinc finger proteins[J]. Current Opinion in Structural Biology, 2005, 15: 367-373
[17]  Bateman A, Coin L, Durbin R, Finn R D, Hollich V, Griffiths-Jones S, Khanna A, Marshall M, Moxon S, Sonnhammer E L, Studholme D J, Yeats C, Eddy S R. The Pfam protein families database[J]. Nucleic Acids Research, 2008, 36(Database issue): 281-288
[18]  Finn R D, Clements J, Eddy S R. HMMER web server: interactive sequence similarity searching[J]. Nucleic Acids Research, 2011, 39(Web Server issue): 29-37
[19]  Letunic I, Doerks T, Bork P. SMART 7: recent updates to the protein domain annotation resource[J]. Nucleic Acids Research, 2012, 40(Database issue): 302-305
[20]  郭安源, 朱其慧, 陈新, 罗静初. GSDS: 基因结构显示系统[J]. 遗传, 2007, 29(8): 1023-1026
[21]  Bailey T L, Boden M, Buske F A, Frith M, Grant C E, Clementi L, Ren J, Li W W, Noble W S. MEME SUITE: tools for motif discovery and searching[J]. Nucleic Acids Research, 2009, 37(Web Server issue): 202-208
[22]  Kumar S, Tamura K, Nei M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics, 2004, 5: 150-163
[23]  Deng H, Liu H, Li X, Xiao J, Wang S. A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease[J]. Plant Physiology, 2012, 158(2): 876-889
[24]  Liang J, Song W, Tromp G, Kolattukudy P E, Fu M. Genome-wide survey and expression profiling of CCCH-zinc finger family reveals a functional module in macrophage activation[J]. PLoS One, 2008, 3(8): 2880
[25]  Wang D, Guo Y, Wu C, Yang G, Li Y, Zheng C. Genome-wide analysis of CCCH zinc finger family in Arabidopsis and rice[J]. BMC Genomics, 2008, 9: 44
[26]  Peng X, Zhao Y, Cao J, Zhang W, Jiang H, Li X, Ma Q, Zhu S, Cheng B. CCCH-type zinc finger family in maize: genome-wide identification, classification and expression profiling under abscisic acid and drought treatments[J]. PLoS One, 2012, 7(7): e40120
[27]  Chai G, Hu R, Zhang D, Qi G, Zuo R, Cao Y, Chen P, Kong Y, Zhou G. Comprehensive analysis of CCCH zinc finger family in poplar (Populus trichocarpa)[J]. BMC Genomics, 2012, 13(1): 253

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133