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核农学报  2013 

小麦FeSOD基因的克隆与序列分析

DOI: 10.11869/hnxb.2013.08.1111, PP. 1111-1117

Keywords: 小麦,FeSOD,克隆,序列分析

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Abstract:

超氧化物歧化酶(superoxidedismutase,SOD)是一种广泛存在于真核生物中的金属酶类,在植物的抗逆性中起到重要的作用。本文以小麦烟农19号为材料,提取植株叶片总RNA,根据NCBI公布的FeSOD基因序列设计简并引物,采用RT-PCR和RACE技术首次获得小麦抗逆基因FeSOD(GenBank:JX398977)的全长cDNA序列。生物学软件分析表明,该序列全长为1271bp,5'UTR为187bp,3'UTR为490bp,开放阅读框长度为591bp,编码197个氨基酸,分子质量为22.8KD,等电点(PI)为5.382,经BlastP和Clustalx进行比对发现,小麦FeSOD的氨基酸序列与玉米、水稻等物种具有高的同源性;同时,利用Mega5软件进行系统谱系分析,发现小麦FeSOD与玉米和水稻的亲缘关系最近。

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