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核农学报  2013 

三价病毒外壳蛋白基因介导的百合抗病毒初步研究

DOI: 10.11869/hnxb.2013.09.1293, PP. 1293-1301

Keywords: 百合,抗病毒,外壳蛋白基因,转基因

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Abstract:

百合病毒病是制约百合生产的一类重要病害,由于抗病资源的匮乏,利用常规育种方法培育抗病毒百合品种的工作几科停滞。本研究克隆了三种常见和危害最严重的百合病毒外壳蛋白(coatprotein,CP)基因,百合无症病毒(Lilysymptomlessvirus,LSV),百合斑驳病毒(Lilymottlevirus,LMoV)和黄瓜花叶病毒(Cucumbermosaicvirus,CMV),并且构建了三价表达载体,用根癌农杆菌(Agrobacteriumtumefaciens)介导的方法转化白天堂百合(Liliumspp.),为百合的抗病毒育种提供种植资源和育种材料。

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