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核农学报  2013 

玉米ZmPEPC基因的克隆及其转化春小麦的研究

DOI: 10.11869/hnxb.2013.11.1617, PP. 1617-1623

Keywords: 玉米,春小麦,ZmPEPC,基因克隆,转化

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Abstract:

磷酸烯醇式丙酮酸羧化酶(PEPC)是C4光合途径中的关键酶,为了研究该酶对春小麦光合特性的改良作用,本研究从玉米(Zeamays)中克隆了ZmPEPC基因,生物信息学分析表明该基因CDS全长2913bp,编码的多肽链包含970个氨基酸,其中175-970位氨基酸组成了磷酸烯醇式丙酮酸羧化酶的功能结构域,在173-184、597-609位具有两个磷酸烯醇式丙酮酸羧化酶活性位点。将该基因通过基因枪法转化优质强筋小麦新春26号,对转基因春小麦的叶绿素含量及磷酸烯醇式丙酮酸羧化酶活性进行测定,结果显示转基因春小麦两项指标较非转基因春小麦均有提高,表明ZmPEPC基因对于春小麦光合特性的改良具有积极的作用,为C4光合途径关键酶在春小麦高产改良中的应用奠定了良好的基础。

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