全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
核农学报  2013 

空间环境诱发玉米胚乳突变体的鉴定与分析

DOI: 10.11869/hnxb.2013.11.1603, PP. 1603-1609

Keywords: 玉米,空间诱变,胚乳突变体,遗传分析,分子检测

Full-Text   Cite this paper   Add to My Lib

Abstract:

用返回式卫星"实践八号"搭载玉米自交系08-641和18-599,从诱变后代中获得籽粒皱缩透明和皱缩不透明两种胚乳突变体,经多代自交获得突变性状稳定的遗传材料。籽粒可溶性糖含量测定结果显示,籽粒皱缩透明突变体st1在授粉10d后籽粒糖含量开始上升,到授粉25d时达到峰值,且明显高于自交系08-641;籽粒皱缩不透明突变体so1籽粒糖含量在授粉10d后开始缓慢上升,到授粉20d时达到峰值,且明显高于自交系18-599。遗传分析和等位性测定结果表明两种突变性状均由单隐性基因控制,籽粒皱缩透明突变基因与su1等位,籽粒皱缩不透明突变基因与sh2等位。DNA序列比对发现,突变体st1在su1基因的第2和第13外显子处发生了单碱基突变,突变体so1在sh2基因的第2、5和16外显子处发生单碱基突变,导致编码氨基酸序列变异,推测这些碱基的突变有可能导致淀粉合成代谢受阻,致使籽粒胚乳糖含量提高。

References

[1]  胡建广,王子明,李余亮,刘建华.我国甜玉米育种研究概况与发展方向[J].玉米科学, 2004, 12(1): 12-15
[2]  姚文华,韩学莉,汪燕芬,谭静,徐春霞,陈洪梅,番兴明.我国甜玉米育种研究现状与发展对策[J].中国农业科技导报, 2011, 13(2): 1-8
[3]  杨泉女,王蕴波.甜玉米胚乳突变基因的研究进展及其在育种中应用的策略[J].分子植物育种, 2005, 3(6): 877-882
[4]  王娜,史振声,王志斌,李凤海,刘峰.甜玉米品质研究进展[J].玉米科学, 2007, 15(6): 47-50
[5]  温贤芳,张龙,戴维序,李春华.天地结合开展我国空间诱变育种研究[J].核农学报, 2004, 18(4): 241-246
[6]  密士军,郝再彬.航天诱变育种研究的新进展[J].黑龙江农业科学, 2002, (4): 31-33
[7]  刘录祥,郑企成.空间诱变与作物改良[M].北京:原子能出版社,1997
[8]  刘录祥,赵林姝,郭会君.作物航天育种研究现状与展望[J].中国农业科技导报, 2007, 9(2): 26-29
[9]  潘光辉,尹贤贵,杨琦凤,张赞.作物航天诱变育种研究进展[J].西南农业学报, 2005, 18(6): 853-857
[10]  曹墨菊,荣廷昭,潘光堂.首例航天诱变玉米雄性不育突变体的遗传分析[J].遗传学报, 2003, 30(9): 817-822
[11]  曹墨菊,荣廷昭,潘光堂.卫星搭载获得玉米基因雄性不育的初步鉴定[J].四川农业大学学报, 2000, 18(2): 100-103
[12]  李玉玲,余永亮,刘艳霞,李学慧,付家锋.两份太空诱变玉米雄性不育突变体的遗传研究[J].遗传, 2007, 29(6): 738-744
[13]  张采波,袁国钊,汪静,潘光堂,荣廷昭,曹墨菊.空间环境诱发玉米细胞质雄性不育突变体的遗传分析[J].遗传, 2011, 33(2): 175-181
[14]  邱正高,杨华,祁志云,袁亮,柯剑鸿,张亚勤.航空诱变的糯玉米矮秆突变体株高与叶片生长分析[J].西南农业学报, 2009, 22(4): 901-904
[15]  Dingas J R, Colleoni C, Myers A M, James M G. Molecular structure of three mutations at the maize surgary1 locus and their allele-specific phenotypic effects[J]. Plant Physiology, 2001, 125(3): 1406-1418
[16]  谭彩霞,封超年,陈静,郭静,郭文善,朱新开,李春燕,彭永欣.作物淀粉合成关键酶及其基因表达的研究进展[J].麦类作物学报, 2008, 28 (5): 912-919
[17]  Pan D, Nelson O E. A debranching enzyme deficiency in endosperms of the sugary-1 mutants of maize[J]. Plant Physiol, 1984, 74(2): 324-328
[18]  Bhave M R, Lawrence S, Barton C, Hannah L C. Identification and molecular characterization of shrunken-2 cDNA clones of maize[J]. The Plant Cell, 1990, 2: 581-588
[19]  Lou S K, Girgux M J, Brenttl V, Vallejos C E, Hannah L C. The maize genome contains a helitron insertion[J]. The Plant Cell, 2003, 15(2): 381-391
[20]  James M G, Robertson D S, Myers A M. Characterization of the maize gene sugary1, a determinant of starch composition in kernels[J]. The Plant Cell, 1995, 7: 417-429
[21]  Lou S, Choi J H, Hannah L C. The AG dinucleotide terminating introns is important but not always required for pre-mRNA splicing in the maize endosperm[J]. Plant Physiology, 1999, 120: 65-72
[22]  Hannah L C, Nelson O E. Characterization of ADP-glucose pyrophosphorylase from shrunken-2 and Brittle-2 mutants of maize[J]. Biochemical Genetics, l976, 14(7-8): 547-560
[23]  Greene T W, Hannah L C. Maize endosperm ADP-glucose pyrophosphorylase SHRUNKEN2 and BRITTLE2 subunit interactions[J]. The Plant Cell, 1998, 10(8): 1295-1306
[24]  Bhave M R, Lawrence S, Barton C, Hannah L C. Identification and molecular characterization of shrunken-2 cDNA clones of maize[J]. The Plant Cell, 1990, 2: 581-588
[25]  Greene T W, Hannah L C. Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is gained through mutants that alter subunit interactions[J]. Proceeding of the National Academy of Sciences, 1998, 95(22):13342-13347
[26]  杨若飞.玉米胚乳突变体的诱导与鉴定[D].成都: 四川农业大学, 2011
[27]  Lou S K, Girgux M J, Brenttl V, Vallejos C E, Hannah L C. The maize genome contains a helitron insertion[J]. The Plant Cell, 2003, 15(2): 381-391
[28]  Lou S, Choi J H, Hannah L C. The AG dinucleotide terminating introns is important but not always required for pre-mRNA splicing in the maize endosperm[J]. Plant Physiology, 1999, 120: 65-72
[29]  王晶英.植物生理生化实验技术与原理[M].哈尔滨:东北林业大学出版社, 2003
[30]  黄培堂.分子克隆实验指南(第三版)[M].北京:科学出版社,2002
[31]  Dingas J R, Colleoni C, Myers A M, James M G. Molecular structure of three mutations at the maize surgary1 locus and their allele-specific phenotypic effects[J]. Plant Physiology, 2001, 125(3): 1406-1418
[32]  谭彩霞,封超年,陈静,郭静,郭文善,朱新开,李春燕,彭永欣.作物淀粉合成关键酶及其基因表达的研究进展[J].麦类作物学报, 2008, 28 (5): 912-919
[33]  Pan D, Nelson O E. A debranching enzyme deficiency in endosperms of the sugary-1 mutants of maize[J]. Plant Physiol, 1984, 74(2): 324-328
[34]  王晶英.植物生理生化实验技术与原理[M].哈尔滨:东北林业大学出版社, 2003
[35]  James M G, Robertson D S, Myers A M. Characterization of the maize gene sugary1, a determinant of starch composition in kernels[J]. The Plant Cell, 1995, 7: 417-429
[36]  黄培堂.分子克隆实验指南(第三版)[M].北京:科学出版社,2002
[37]  Hannah L C, Nelson O E. Characterization of ADP-glucose pyrophosphorylase from shrunken-2 and Brittle-2 mutants of maize[J]. Biochemical Genetics, l976, 14(7-8): 547-560
[38]  Greene T W, Hannah L C. Maize endosperm ADP-glucose pyrophosphorylase SHRUNKEN2 and BRITTLE2 subunit interactions[J]. The Plant Cell, 1998, 10(8): 1295-1306
[39]  Greene T W, Hannah L C. Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is gained through mutants that alter subunit interactions[J]. Proceeding of the National Academy of Sciences, 1998, 95(22):13342-13347
[40]  杨若飞.玉米胚乳突变体的诱导与鉴定[D].成都: 四川农业大学, 2011

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133