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核农学报  2013 

水稻Pi-ta启动子的克隆及其功能分析

DOI: 10.11869/hnxb.2013.12.1803, PP. 1803-1808

Keywords: Pi-ta,启动子,GUS,转基因水稻

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Abstract:

本研究分离了水稻品种日本晴Pi-ta基因的启动子,并构建其GUS融合表达载体,通过农杆菌介导的遗传转化获得了其转基因植株。以获得的转基因水稻为材料进行GUS组织染色和GUS(β-葡萄糖苷酸酶)酶活性分析,结果表明,GUS基因在根、茎和叶中都有表达,转基因植株在接种稻瘟病菌后GUS活性显著增加。用抗病信号分子水杨酸(SA)和茉莉酸甲酯(MeJA)处理后,Pi-ta启动子驱动的GUS活性也显著增加。以上结果表明,Pi-ta启动子是一个对稻瘟病菌应答的诱导型启动子,同时Pi-ta启动子还受到SA和MeJA的诱导表达。

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