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核农学报  2014 

水稻千粒重QTLqtgw1基因的精细定位

DOI: 10.11869/j.issn.100-8551.2014.02.0217, PP. 217-223

Keywords: 水稻,数量性状座位,千粒重,精细定位,qtgw1

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Abstract:

粒重是水稻产量性状的主要构成因子及影响稻米品质的重要因素之一。本研究针对第1染色体上对千粒重具有重要作用的QTL进行精细定位。利用从珍汕97/密阳46重组自交系高代群体中挑选出在第1染色体短臂RM151-RM10404、RM151-RM10425和RM1344-RM5359区间呈杂合而背景基本纯合的5个剩余杂合体(ResidualHeterozygousLine,RHL),衍生5个F2:3群体(RHL群体),对千粒重QTL进一步分析,在RM151-RM1344区间检测到控制千粒重的QTLqtgw1,其增效等位基因均来自母本珍汕97。应用SSR标记检测,从其中一个RHL衍生群体中筛选到分离区间为RM151-RM3746,RM151-RM10402和RM10381-RM10425的4个单株,衍生4个F2群体(FF群体),利用该群体将QTLqtgw1定位在RM10376-RM10404之间。从其中一个F2群体筛选到分离区间为RM10381-RM10402,RM10390-RM10425和RM1344-RM10425的3个单株,衍生3个F2群体(L群体),根据3个L群体QTL分析结果,将千粒重QTLqtgw1定位在RM10381-RM10404,物理距离约246.6kb。

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